Reduced expression of CD44 on monocytes and neutrophils in systemic lupus erythematosus: relations with apoptotic neutrophils and disease activity
Reduced expression of CD44 on monocytes and neutrophils in systemic lupus erythematosus: relations with apoptotic neutrophils and disease activity
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DOI：
10.1136/ard.60.10.950
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发表时间：
2001-10-01
影响因子：
27.4
通讯作者：
Bell, AL
中科院分区：
文献类型：
--
作者：
Cairns, AP;Crockard, AD;Bell, AL
背景-在系统性红斑狼疮 (SLE) 中，已证实凋亡中性粒细胞数量增加以及凋亡细胞的单核细胞/巨噬细胞清除受损。 CD44参与凋亡性中性粒细胞的清除。目的——测定SLE患者外周血单核细胞和中性粒细胞上CD44的表达，并探讨其与疾病活动度和循环凋亡中性粒细胞数量的关系。方法——采集31例SLE患者、19例健康正常人和19例类风湿性关节炎（RA）患者的外周血。通过免疫荧光标记和流式细胞术测定单核细胞和中性粒细胞表面CD44表达的密度，结果表示为平均通道荧光（MCF）值。通过形态学测量 15 名 SLE 患者、9 名 RA 患者和 6 名正常受试者中的中性粒细胞凋亡。 结果：SLE 患者的单核细胞 CD44 表达（中位 MCF 4.71）显着低于健康正常受试者（中位 MCF 5.61）和 RA 对照（中位 MCF 5.39）。 SLE 患者的中性粒细胞 CD44 表达（中位 MCF 1.95）也显着低于健康正常受试者（中位 MCF 2.37）和 RA 对照（中位 MCF 2.60）。单核细胞而非中性粒细胞的 CD44 表达与凋亡中性粒细胞的百分比呈负相关。 SLE中单核细胞或中性粒细胞CD44表达与疾病活动或损害没有显着相关性。结论-SLE中单核细胞和中性粒细胞CD44表达降低，这可能导致单核细胞来源的巨噬细胞对凋亡中性粒细胞的识别和清除受损。
Background-Increased numbers of apoptotic neutrophils, and impaired monocyte/macrophage clearance of apoptotic cells, have been demonstrated in systemic lupus erythematosus (SLE). CD44 is implicated in the clearance of apoptotic neutrophils.Objective-To determine the expression of CD44 on peripheral blood monocytes and neutrophils in SLE, and examine the relations with disease activity and numbers of circulating apoptotic neutrophils.Methods-Peripheral blood was sampled from 31 patients with SLE, 19 healthy normal subjects, and 19 patients with rheumatoid arthritis (RA). Monocyte and neutrophil density of surface CD44 expression was determined by immunofluorescence labelling and flow cytometry, and results expressed as mean channel fluorescence (MCF) values. Neutrophil apoptosis was measured by morphology in 15 patients with SLE, nine with RA, and six normal subjects.Results-Monocyte CD44 expression was significantly lower in SLE (median MCF 4.71) than in healthy normal subjects (median MCF 5.61) and controls with RA (median MCF 5.39). Neutrophil CD44 expression was also significantly lower in SLE (median MCF 1.95) than in healthy normal subjects (median MCF 2.37) and controls with RA (median MCF 2.60). Monocyte, but not neutrophil, CD44 expression correlated negatively with the percentage of apoptotic neutrophils. There was no significant correlation of monocyte or neutrophil CD44 expression in SLE with disease activity or damage.Conclusions-Monocyte and neutrophil CD44 expression is reduced in SLE, and this may contribute to the impaired recognition and clearance of apoptotic neutrophils by monocyte derived macrophages.