Effects on larval metamorphosis in the mussel Mytilus coruscus of compounds that act on downstream effectors of G-protein-coupled receptors
Effects on larval metamorphosis in the mussel Mytilus coruscus of compounds that act on downstream effectors of G-protein-coupled receptors
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作用于 G 蛋白偶联受体下游效应子的化合物对贻贝幼虫变态的影响
DOI：
10.1017/s0025315416001417
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发表时间：
2018-03-01
影响因子：
1.2
通讯作者：
Yang, Jin-Long
中科院分区：
文献类型：
--
作者：
Liang, Xiao;Chen, Yu-Ru;Yang, Jin-Long
在实验室条件下，研究了贻贝（Mytilus coruscus）幼虫对影响G蛋白和腺苷酸环化酶/环腺苷酸（AC/cAMP）途径的药物的变态反应。G蛋白激活剂鸟苷5 '-[γ-亚氨基]三磷酸三钠盐水合物和鸟苷5'-[β-硫代]三磷酸四锂盐在连续暴露试验中仅诱导幼虫变态，而G蛋白抑制剂鸟苷5-[-γ-硫代]二磷酸三锂盐没有表现出诱导活性。在连续暴露试验中，非特异性磷酸二酯酶抑制剂茶碱和cAMP特异性磷酸二酯酶IV抑制剂4-（3-丁氧基-4-甲氧基苄基）咪唑烷-2-酮显示出诱导活性，而非特异性磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤仅在10（-4）M下显示出诱导活性。环核苷酸类似物N-6，2 ′-O-二丁酰腺苷3 ′，5 ′-环单磷酸钠盐没有表现出显著的诱导活性。在连续暴露试验中，腺苷酸环化酶激活剂毛喉素和腺苷酸环化酶抑制剂硝基咪唑在10（-4）至10（-3）M浓度下均表现出诱导活性。其中腺苷酸环化酶抑制剂（+/-）-咪康唑硝酸盐的诱导效果最好。这些结果表明，G蛋白偶联受体和AC/cAMP信号转导途径可能介导该物种幼虫的变态。
The metamorphic responses of mussel (Mytilus coruscus) larvae to pharmacological agents affecting G proteins and the adenylate cyclase/cyclic AMP (AC/cAMP) pathway were examined in the laboratory. The G protein activators guanosine 5'-[gamma-imido]triphosphate trisodium salt hydrate and guanosine 5'-[beta-thio]triphosphate tetralithium salt only induced larval metamorphosis in continuous exposure assays, and the G protein inhibitor guanosine 5-[-gamma-thio]diphosphate trilithium salt did not exhibit inducing activity. The non-specific phosphodiesterase inhibitor theophylline and the cAMP-specific phosphodiesterase IV inhibitor 4-(3-Butoxy-4-methoxybenzyl)imidazolidin-2-one exhibited inducing activity, while the non-specific phosphodiesterase inhibitor 3-Isobutyl-1-methylxanthine only showed inducing activity at 10(-4) M in continuous exposure assays. The cyclic nucleotide analogue N-6,2'-O-Dibutyryladenosine 3',5'-cyclic monophosphate sodium salt did not exhibit significant inducing activity. Both the adenylate cyclase activator forskolin and the adenylate cyclase inhibitor nitroimidazole exhibited inducing activity at 10(-4) to 10(-3) M concentrations in continuous exposure assays. Among these tested agents, the adenylate cyclase inhibitor (+/-)-miconazole nitrate salt showed the most promising inducing effect. The present results indicate that G protein-coupled receptors and signal transduction by AC/cAMP pathway could mediate metamorphosis of larvae in this species.