Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A 1H-NMR-based metabonomic study.
Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A 1H-NMR-based metabonomic study.
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DOI：
10.1371/journal.pone.0173213
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Xu JP
中科院分区：
文献类型：
--
作者：
Deng MJ;Lin XD;Wen CW;Dong MJ;Lin QT;Zhang SZ;Xu JP
1-脱氧野尻霉素（DNJ）是一种天然的D-葡萄糖类似物，在体内对α-葡萄糖苷酶有很强的抑制作用。DNJ在小鼠或其他哺乳动物体内的抗糖尿病作用已被广泛研究，但其在昆虫体内的生理和毒性作用却鲜有报道。本研究以蓖麻蚕四龄幼虫中肠为材料，研究了DNJ的生物学效应。利用核磁共振（NMR）代谢组学技术，分析了中肠糖代谢、脂质代谢和能量代谢途径的变化。蓖麻蚕幼虫是由DNJ引起的。模式识别分析（偏最小二乘-判别分析，PLS-DA）结果表明，0.25%DNJ、0.5%DNJ和0.5%DNJ与胶乳（1：1）混合物4组与对照组差异显著。此外，还鉴定了中肠中DNJ介导的调节的几种代谢途径。与对照组相比，0.5%DNJ组、乳胶组和混合组的丙氨酸、琥珀酸、谷氨酸和富马酸含量均降低，2个DNJ组的胆碱含量均升高，海藻糖含量均升高。因此，这些结果表明DNJ通过限制磷脂代谢的水解途径来调节脂质代谢。此外，DNJ通过抑制海藻糖的水解、糖酵解和三羧酸（TCA）循环，对能量代谢具有潜在的负面影响。总的来说，DNJ作为单一成分，是调节脂质代谢和抑制能量代谢的有效物质。
1-deoxynojirimycin (DNJ) is a natural D-glucose analogue and has a strong physiological activity in inhibiting α-glucosidase in vivo. The antidiabetic effects of DNJ in mice or other mammals were extensively explored, but the physiological and toxic roles of DNJ in insects was seldom reported. In this study, the biological effects of DNJ were examined in midgut extracts of fourth-instar larvae of Eri silkworm (Samia cynthia ricini, Saturniidae). Based on nuclear magnetic resonance (NMR) metabonomics technology, we analyzed the alterations of glycometabolism, lipids, and energy metabolism pathways in the midgut of S. cynthia ricini caused by DNJ. Pattern recognition analysis (partial least square-discriminant analysis, PLS-DA) showed that four groups of latex, 0.25% DNJ, 0.5% DNJ and the mixture of 0.5% DNJ and latex (1:1) were distinctly different from the control group. Moreover, several metabolic pathways of DNJ-mediated modulation in the midgut were identified. Compared with the control group, alanine, succinate, glutamate, and fumarate concentrations decreased in three groups of 0.5% DNJ, latex, and the mixture, choline levels increased in two DNJ groups, and trehalose levels increased in all experimental groups. Therefore, these results suggest that DNJ modulated lipid metabolism by limiting the hydrolysis pathways of phospholipids metabolism. Additionally, DNJ has a potent negative effect on energy metabolism by inhibiting the hydrolysis of trehalose, glycolysis and the tricarboxylic acid (TCA) cycle. Overall, DNJ, as a single-ingredient, is an efficient substance for modulating lipid metabolism and inhibiting energy metabolism.