The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins
The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins
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DOI：
10.1083/jcb.201102044
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发表时间：
2011-08-08
影响因子：
7.8
通讯作者：
Pfanner, Nikolaus
中科院分区：
文献类型：
--
作者：
Becker, Thomas;Wenz, Lena-Sophie;Pfanner, Nikolaus
线粒体外膜含有转运酶复合物，用于前体蛋白的输入。外膜复合体的转位酶作为一般的前蛋白进入门，而分选和组装机械复合体介导外膜β -桶蛋白的膜插入。已知几种α -螺旋外膜蛋白携带多个跨膜片段；然而，只有有限的信息是可利用的这些蛋白质的生物发生。我们报道了缺乏线粒体输入蛋白1 （Mim1）的线粒体在多跨外膜蛋白的生物发生中受损，而Mim1的过表达刺激其输入。Mim1复合体与受体Tom70合作结合前体蛋白并促进其插入和组装到外膜。我们得出结论，Mim1复合体在基因的导入中起着核心作用。-具有多个跨膜片段的螺旋外膜蛋白。
The mitochondrial outer membrane contains translocase complexes for the import of precursor proteins. The translocase of the outer membrane complex functions as a general preprotein entry gate, whereas the sorting and assembly machinery complex mediates membrane insertion of beta-barrel proteins of the outer membrane. Several alpha-helical outer membrane proteins are known to carry multiple transmembrane segments; however, only limited information is available on the biogenesis of these proteins. We report that mitochondria lacking the mitochondrial import protein 1 (Mim1) are impaired in the biogenesis of multispanning outer membrane proteins, whereas overexpression of Mim1 stimulates their import. The Mim1 complex cooperates with the receptor Tom70 in binding of precursor proteins and promotes their insertion and assembly into the outer membrane. We conclude that the Mim1 complex plays a central role in the import of.-helical outer membrane proteins with multiple transmembrane segments.