A Digital Microfluidic Approach to Proteomic Sample Processing
A Digital Microfluidic Approach to Proteomic Sample Processing
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DOI：
10.1021/ac900522a
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发表时间：
2009-06-01
影响因子：
7.4
通讯作者：
Wheeler, Aaron R.
中科院分区：
文献类型：
--
作者：
Luk, Vivienne N.;Wheeler, Aaron R.
蛋白质组学分析的一个共同特点是需要广泛的生化处理。数字微流体（DMF），一种技术，其特征在于离散微滴的操作;（100 nL-10 μ L）的电极的开放阵列上，是一个很好的匹配进行快速，自动化的溶液相反应。在这里，我们报告了一种基于DMF的方法，整合了蛋白质组学中的几个常见处理步骤，包括还原，烷基化和酶消化。荧光分析用于定量评价每个反应步骤的动力学和再现性，并且MALDI-MS用于定性确认。该方法快速、简便、重复性好，有望成为蛋白质组学研究的新工具。
A common characteristic for proteomic analyses is the need for extensive biochemical processing. Digital microfluidics (DMF), a technique characterized by the manipulation of discrete microdroplets; (100 nL-10 mu L) on an open array of electrodes, is a good match for carrying out rapid, automated solution-phase reactions. Here, we report a DMF-based method integrating several common processing steps in proteomics, including reduction, alkylation, and enzymatic digestion. Fluorogenic assays were used to quantitatively evaluate the kinetics and reproducibility of each reaction step, and MALDI-MS was used for qualitative confirmation. The method is fast, facile, and reproducible, and thus has the potential to be a useful new tool in proteomics.