Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels.
Quantitative [Fe]MRI of PSMA-targeted SPIONs specifically discriminates among prostate tumor cell types based on their PSMA expression levels.
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DOI：
10.2147/ijn.s93409
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发表时间：
2016
影响因子：
8
通讯作者：
Sillerud LO
中科院分区：
文献类型：
--
作者：
Sillerud LO
我们报告了一种称为 [Fe]MRI（发音为 fem-ree）的通用理论的开发、实验验证和应用，该理论用于对生物组织和其他部位中添加的磁性纳米粒子或其他磁性造影剂进行非侵入性定量分子磁共振成像 (MRI)。 [Fe]MRI 可以轻松地在任何 MRI 仪器上实现，只需测量感兴趣组织的背景核磁弛豫时间 (T1、T2)、注射磁性颗粒，以及随后采集一对 T1 加权和 T2 加权图像。将这些图像转换为对比度图像，减去这些图像以产生与绝对纳米粒子、铁浓度（[Fe]）图像成比例的对比度差值图像。 [Fe]MRI 使用琼脂糖凝胶中的超顺磁性氧化铁纳米颗粒 (SPION) 样品和与人前列腺肿瘤细胞结合的样品进行了验证。 [Fe]MRI 测量体外结合的抗前列腺特异性膜抗原 (PSMA) 的 SPION 与 PSMA 阳性 LNCaP 和 PSMA 阴性 DU145 细胞的结合，可以根据 PSMA 表达水平轻松区分前列腺肿瘤细胞类型。 SPION 的低 [Fe] 检测限约为 2 μM，可对浓度远低于美国食品和药物管理局的人体铁剂量指南（<90 μM，5 mg/kg）的添加铁进行灵敏的 MRI。
We report the development, experimental verification, and application of a general theory called [Fe]MRI (pronounced fem-ree) for the non-invasive, quantitative molecular magnetic resonance imaging (MRI) of added magnetic nanoparticles or other magnetic contrast agents in biological tissues and other sites. [Fe]MRI can easily be implemented on any MRI instrument, requiring only measurements of the background nuclear magnetic relaxation times (T1, T2) of the tissue of interest, injection of the magnetic particles, and the subsequent acquisition of a pair of T1-weighted and T2-weighted images. These images, converted into contrast images, are subtracted to yield a contrast difference image proportional to the absolute nanoparticle, iron concentration, ([Fe]) image. [Fe]MRI was validated with the samples of superparamagnetic iron oxide nanoparticles (SPIONs) both in agarose gels and bound to human prostate tumor cells. The [Fe]MRI measurement of the binding of anti-prostate specific membrane antigen (PSMA) conjugated SPIONs to PSMA-positive LNCaP and PSMA-negative DU145 cells in vitro allowed a facile discrimination among prostate tumor cell types based on their PSMA expression level. The low [Fe] detection limit of ~2 μM for SPIONs allows sensitive MRI of added iron at concentrations considerably below the US Food and Drug Administration’s human iron dosage guidelines (<90 μM, 5 mg/kg).