Different Interspecies Electron Transfer Patterns during Mesophilic and Thermophilic Syntrophic Propionate Degradation in Chemostats
Different Interspecies Electron Transfer Patterns during Mesophilic and Thermophilic Syntrophic Propionate Degradation in Chemostats
复制标题
恒化器中嗜温和嗜热同养丙酸盐降解过程中不同种间电子转移模式
DOI：
10.1007/s00248-020-01485-x
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发表时间：
2020-07-01
影响因子：
3.6
通讯作者：
Tang, Yue-Qin
中科院分区：
文献类型：
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作者：
Chen, Ya-Ting;Zeng, Yan;Tang, Yue-Qin
丙酸盐是有机废物厌氧消化生成CO2和CH4的主要中间产物之一。在产甲烷环境中，丙酸通过共生的丙酸氧化菌和产甲烷菌之间的互惠相互作用而降解。虽然温度严重影响微生物生态和产甲烷过程的性能，其对丙酸降解过程中的互养相互作用的影响仍然知之甚少。在这项研究中，采用元基因组学和元转录组学来比较嗜温和嗜热丙酸盐降解群落。甲烷丙酸降解涉及多种互养生物（互养菌属、Smithella和互养单胞菌属）、途径、相互作用和对甲酸盐基电子转移至产甲烷伴侣（即，Methanoculleus）。在嗜热丙酸盐降解中，一种互养生物占主导地位（Pelotomaculum），种间H2转移起主要作用，并且存在遗传和代谢多样的H2氧化产甲烷菌（即，甲烷球菌属、甲烷热杆菌属和甲烷嗜热球菌属）。本研究表明，微生物的相互作用，代谢途径和生态位多样性是不同的嗜温和嗜热微生物群落负责互养丙酸降解。
Propionate is one of the major intermediates in anaerobic digestion of organic waste to CO2and CH4. In methanogenic environments, propionate is degraded through a mutualistic interaction between symbiotic propionate oxidizers and methanogens. Although temperature heavily influences the microbial ecology and performance of methanogenic processes, its effect on syntrophic interaction during propionate degradation remains poorly understood. In this study, metagenomics and metatranscriptomics were employed to compare mesophilic and thermophilic propionate degradation communities. Mesophilic propionate degradation involved multiple syntrophic organisms (Syntrophobacter,Smithella, andSyntrophomonas), pathways, interactions, and preference toward formate-based electron transfer to methanogenic partners (i.e.,Methanoculleus). In thermophilic propionate degradation, one syntrophic organism predominated (Pelotomaculum), interspecies H2transfer played a major role, and phylogenetically and metabolically diverse H2-oxidizing methanogens were present (i.e.,Methanoculleus,Methanothermobacter, andMethanomassiliicoccus). This study showed that microbial interactions, metabolic pathways, and niche diversity are distinct between mesophilic and thermophilic microbial communities responsible for syntrophic propionate degradation.