Strong epigenetic similarity between maize centromeric and pericentromeric regions at the level of small RNAs, DNA methylation and H3 chromatin modifications.
Strong epigenetic similarity between maize centromeric and pericentromeric regions at the level of small RNAs, DNA methylation and H3 chromatin modifications.
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DOI：
10.1093/nar/gkr862
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发表时间：
2012-02
影响因子：
14.9
通讯作者：
Dawe RK
中科院分区：
文献类型：
--
作者：
Gent JI;Dong Y;Jiang J;Dawe RK
着丝粒功能和姐妹染色单体凝聚力都依赖于着丝粒周围染色质结构，与异染色质相关的因子被认为在区分着丝粒和着丝粒周围以及赋予着丝粒周围内在功能方面具有普遍、保守的作用。我们应用全基因组测序方法来定量玉米（Zea mays）中的RNA表达、DNA甲基化和组蛋白修饰分布，重点关注具有几乎完全测序的着丝粒和近着丝粒的两条玉米染色体。除了存在所有着丝粒共有的组蛋白H3变体，着丝粒组蛋白H3（CENH3）之外，我们没有发现RNA表达或染色质修饰可以将近着丝粒与着丝粒或染色体臂明确区分开来，我们也没有鉴定出准确预测CENH3位置的表观遗传特征。RNA表达和染色质修饰频率与距离着丝粒的距离广泛相关，逐渐达到峰值或倾向于武器。当从其他系统的实验数据的背景下解释，我们的研究结果表明，着丝粒可能会赋予必要的功能（如凝聚力保留）侧翼序列，无论当地异染色质配置文件。
Both kinetochore function and sister chromatid cohesion can depend upon pericentromere chromatin structure, and factors associated with heterochromatin have been proposed to have general, conserved roles in distinguishing centromeres and pericentromeres and in conferring pericentromere-intrinsic functions. We applied genome-wide sequencing approaches to quantify RNA expression, DNA methylation and histone modification distributions in maize (Zea mays), focusing on two maize chromosomes with nearly fully sequenced centromeres and pericentromeres. Aside from the presence of the Histone H3 variant common to all centromeres, Centromeric Histone H3 (CENH3), we found no RNA expression or chromatin modifications that clearly differentiate pericentromeres from either centromeres or from chromosome arms, nor did we identify an epigenetic signature that accurately predicts CENH3 location. RNA expression and chromatin modification frequencies were broadly associated with distance from centromeres, gradually peaking or dipping toward arms. When interpreted in the context of experimental data from other systems, our results suggest that centromeres may confer essential functions (such as cohesion retention) to flanking sequence regardless of the local heterochromatin profile.
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影响因子：
4.5
作者：
Du Y;Topp CN;Dawe RK
通讯作者：
Dawe RK
影响因子：
7
作者：
Koo, Dal-Hoe;Han, Fangpu;Jiang, Jiming
通讯作者：
Jiang, Jiming
影响因子：
7.2
作者：
Koo, Dal-Hoe;Jiang, Jiming
通讯作者：
Jiang, Jiming
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
DOI：
10.1073/pnas.0507947103
发表时间：
2006-03-14
影响因子：
11.1
作者：
Lam, AL;Boivin, CD;Sullivan, BA
通讯作者：
Sullivan, BA