Development and evaluation of silk fibroin-based nerve grafts used for peripheral nerve regeneration
Development and evaluation of silk fibroin-based nerve grafts used for peripheral nerve regeneration
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用于周围神经再生的丝素蛋白神经移植物的开发和评价
DOI：
10.1016/j.biomaterials.2007.09.001
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发表时间：
2007-12-01
期刊：
影响因子：
14
通讯作者：
Gu, Xiaosong
中科院分区：
文献类型：
--
作者：
Yang, Yumin;Ding, Fel;Gu, Xiaosong
丝素蛋白（SF）来源于天然丝，长期以来一直用作纺织材料，近年来已成为组织工程应用的重要生物材料。我们以前曾报道过SF纤维与周围神经组织和细胞具有良好的体外生物相容性。在本研究中，我们开发了一种新型仿生设计的SF基神经移植物（SF移植物），该移植物由插入定向SF细丝的SF神经引导导管（NGC）组成。通过成熟的方法制备的SF-NGC表现出蛋壳样的微观结构，这是其上级机械和渗透性能优越，有利于神经再生的原因。将SF移植物用于桥接植入大鼠坐骨神经10 mm长的缺损，并通过电生理评估、FluoroGold逆行追踪和组织学研究相结合来评估植入后6个月周围神经修复的结果。检查的功能和形态学参数表明，SF移植物可以促进周围神经再生，其效果接近通常被认为是治疗大型周围神经缺损的金标准的神经自体移植物，从而提高了使用这些新开发的神经移植物作为有前途的替代神经自体移植物的潜在可能性。(C)2007爱思唯尔有限公司版权所有。
Silk fibroin (SF), derived from natural silk long used as a textile material, has recently become an important biomaterial for tissue engineering applications. We have previously reported on good in vitro biocompatibility of SF fibers with peripheral nerve tissues and cells. In the present study, we developed a novel biomimetic design of the SF-based nerve graft (SF graft) which was composed of a SF-nerve guidance conduit (NGC) inserted with oriented SF filaments. The SF-NGC prepared via well-established procedures exhibits an eggshell-like microstructure that is responsible for its superior mechanical and permeable properties beneficial to nerve regeneration. The SF graft was used for bridge implantation across a 10-mm long sciatic nerve defect in rats, and the outcome of peripheral nerve repair at 6 months post-implantation was evaluated by a combination of electrophysiological assessment, FluoroGold retrograde tracing and histological investigation. The examined functional and morphological parameters show that SF grafts could promote peripheral nerve regeneration with effects approaching those elicited by nerve autografts which are generally considered as the gold standard for treating large peripheral nerve defects, thus raising a potential possibility of using these newly developed nerve grafts as a promising alternative to nerve autografts. (C) 2007 Elsevier Ltd. All rights reserved.