Human granulocytes generated in continuous bone marrow culture are physiologically normal.
Human granulocytes generated in continuous bone marrow culture are physiologically normal.
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DOI：
10.1182/blood.v58.4.724.724
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发表时间：
1981-10
期刊：
影响因子：
20.3
通讯作者：
H. M. Greenberg;P. Newburger;L. Parker;T. Novak;J. Greenberger
中科院分区：
文献类型：
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作者：
H. M. Greenberg;P. Newburger;L. Parker;T. Novak;J. Greenberger
一个长期的骨髓培养系统已被推导出来的维持和增殖的人造血干细胞和粒细胞在体外长达20周。在这些培养物中产生的粒细胞在8周的生理特性，包括吞噬作用，脱粒，呼吸爆发，和细菌杀灭新鲜的人外周血粒细胞相当：在几个培养物中产生的单个粒细胞20周表现出正常的超氧化物生成能力的NBT染料还原玻片试验。因此，在连续骨髓培养中产生的人粒细胞保留了许多与体内杀菌能力相关的生物学功能，并表明该系统在粒细胞分化障碍的研究中应具有价值。
A long-term bone marrow culture system has been derived for maintenance and proliferation of human hemopoietic stem cells and granulocytes in vitro for up to 20 wk. The granulocytes generated in these cultures at 8 wk were comparable to fresh human peripheral blood granulocytes in physiologic properties, including phagocytosis, degranulation, respiratory burst, and bacterial killing: individual granulocytes generated up to 20 wk in several cultures demonstrated normal superoxide-generating capacity by NBT dye reduction slide test. Thus, human granulocytes generated in continuous marrow culture retain many biologic functions associated with bacterocidal capacity in vivo and indicate that this system should be of value in studies of disorders of granulocyte differentiation.