B-LINEAGE SPECIFIC INTERACTIONS OF AN IMMUNOGLOBULIN ENHANCER WITH CELLULAR FACTORS INVIVO
B-LINEAGE SPECIFIC INTERACTIONS OF AN IMMUNOGLOBULIN ENHANCER WITH CELLULAR FACTORS INVIVO
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DOI：
10.1126/science.3917574
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
GILBERT, W
中科院分区：
文献类型：
--
作者：
EPHRUSSI, A;CHURCH, GM;GILBERT, W
小鼠重链免疫球蛋白基因含有组织特异性增强子。通过对活细胞进行硫酸二甲酯保护实验，结合基因组测序，在体内研究了增强子和侧翼序列。相对于裸DNA上的反应，在骨髓瘤、B和早期B细胞的增强子序列内，鸟嘌呤残基对硫酸二甲酯的反应性存在变化（保护和增强），而在非B谱系细胞中几乎没有变化。大多数受影响的残基在四个簇中，在与八聚体5′CAGGTGGC 3′同源的序列中（C，胞嘧啶; A，腺嘌呤; G。鸟嘌呤; T，胸腺嘧啶）。G反应模式的改变与分子与小鼠免疫球蛋白重链增强子的组织特异性结合一致。
The mouse heavy chain immunoglobulin gene contains a tissue-specific enhancer. The enhancer and flanking sequences were studied in vivo by carrying out dimethyl sulfate protection experiments on living cells, in combination with genomic sequencing. Relative to reactions on naked DNA, there are changes (protections and enhancements) in the reactivity of guanine residues to dimethyl sulfate within the enhancer sequence in myeloma, B, and early B cells, whereas virtually no alterations appear in cells of non-B lineage. Most of the affected residues are in four clusters, in sequences homologous to the octamer 5′CAGGTGGC 3′ (C, cytosine; A, adenine; G. guanine; T, thymine). The alterations in the pattern of G reactivity are consistent with the tissue-specific binding of molecules to the mouse immunoglobulin heavy chain enhancer.