Irradiatin effect on osteoclastgenesis stimulated
Irradiatin effect on osteoclastgenesis stimulated
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刺激破骨细胞生成的辐照作用
DOI：
10.1097/hp.0b013e3182118333
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发表时间：
2012
期刊：
影响因子：
2.2
通讯作者：
Masahiko Sawajiri
中科院分区：
文献类型：
--
作者：
M Tsumura;Y Shibukawa;T Muramatsu;U Sobhan;M Sato;H Ichikawa;M Tazaki;Masahiko Sawajiri
为了研究碳离子和伽马射线照射对癌症诱导的破骨细胞发生的影响，我们用辐照和未辐照的MCF7人乳腺癌细胞的条件培养基培养小鼠颅MC3T3-E1细胞。作者检测了条件MCF7培养基处理后成骨细胞MC3T3-E1中RANKL和OPG mRNA的表达。MCF7细胞经条件培养基处理后，MC3T3-E1与骨髓细胞共培养，破骨细胞数量减少。对照MCF7细胞的条件培养基使MC3T3-E1细胞的RANKL/OPG mRNA比值升高；MCF7细胞的碳离子辐照抑制了这种效应。这些数据表明，乳腺癌细胞与MC3T3-E1细胞之间的间接相互作用通过调节RANKL表达诱导体外破骨细胞发生，而这一过程被碳离子照射抑制。
To examine the effects of carbon ion and gamma ray irradiation on cancer-induced osteoclastogenesis, mouse calvaria MC3T3-E1 cells were cultured with conditioned medium from irradiated and non-irradiated MCF7 human breast cancer cells. The authors examined RANKL and OPG mRNA expression in osteoblastic MC3T3-E1 cells following treatment with conditioned MCF7 medium. Co-cultured MC3T3-E1 and bone marrow cells treated with conditioned medium from irradiated MCF7 cells showed decreased numbers of osteoclasts, assessed using TRAP staining. Conditioned medium from control MCF7 cells elevated the RANKL/OPG mRNA ratio in MC3T3-E1 cells; this effect was suppressed by carbon ion irradiation of the MCF7 cells. These data demonstrate that indirect interactions between breast cancer cells and MC3T3-E1 cells induce osteoclastogenesis in vitro through modulation of RANKL expression and that this process is suppressed by carbon ion irradiation.