Hormone Distribution and Transcriptome Profiles in Bamboo Shoots Provide Insights on Bamboo Stem Emergence and Growth
Hormone Distribution and Transcriptome Profiles in Bamboo Shoots Provide Insights on Bamboo Stem Emergence and Growth
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DOI：
10.1093/pcp/pcx023
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发表时间：
2017-04-01
影响因子：
4.9
通讯作者：
Reuscher, Stefan
中科院分区：
文献类型：
--
作者：
Gamuyao, Rico;Nagai, Keisuke;Reuscher, Stefan
生长和发育是生物体一生中紧密协调的事件。在温带的竹子植物中，春天是环境条件适合新芽出现的季节。以前的研究表明，竹子植物经历了一个消耗能量的“茎快速生长”阶段。然而，对竹茎伸长起始过程的了解甚少。为了了解竹茎生长的开始，我们对毛竹（Phyllostachys edulis）新伸长芽的组织区域进行了激素和转录组分析。生长激素生长素、细胞分裂素和赤霉素集中在茎尖，胁迫激素ABA、水杨酸（SA）和茉莉酸（JA）集中在茎下部。茎的成熟基部显示出与细胞壁代谢和苯基丙烷代谢产物（如木质素）的生物合成相关的转录本的富集。在幼嫩的上茎区，细胞形成和DNA合成相关基因的表达丰富。此外，茎尖区与分生组织维持、叶片分化发育、轴向极性和开花有关的基因表达增加。我们的研究结果整合了竹茎生长初期激素和转录组程序的空间调节。
Growth and development are tightly co-ordinated events in the lifetime of living organisms. In temperate bamboo plants, spring is the season when environmental conditions are suitable for the emergence of new shoots. Previous studies demonstrated that bamboo plants undergo an energy-consuming 'fast stem growth' phase. However, the events during the initiation of stem elongation in bamboo are poorly understood. To understand the onset of bamboo stem growth, we performed hormone and transcriptome profiling of tissue regions in newly elongating shoots of the Moso bamboo Phyllostachys edulis. The growth hormones auxins, cytokinins and gibberellins accumulated in the shoot apex, while the stress hormones ABA, salicylic acid (SA) and jasmonic acid (JA) are predominantly found in the lower part of the stem. The mature basal part of the stem showed enrichment of transcripts associated with cell wall metabolism and biosynthesis of phenylpropanoid metabolites, such as lignin. In the young upper stem region, expression of cell formation-and DNA synthesis-related genes was enriched. Moreover, the apical region showed enhanced expression of genes involved in meristem maintenance, leaf differentiation and development, abaxial/adaxial polarity and flowering. Our findings integrate the spatial regulation of hormones and transcriptome programs during the initiation of bamboo stem growth.