Production of Fumaric Acid from Raw Starchy Materials by Rhizopus oryzae with SSF
Production of Fumaric Acid from Raw Starchy Materials by Rhizopus oryzae with SSF
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米根霉SSF从淀粉原料生产富马酸
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
李霜
中科院分区：
文献类型：
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作者：
高敏;徐晓康;徐晴;李霜
为降低发酵法生产富马酸的生产成本，对木薯粉、玉米粉、脱胚玉米粉和淀粉4种淀粉原料进行了研究。通过对不同原料中氮含量的比较，得出原料碳氮比是影响富马酸发酵的关键因素。在此基础上，选择脱胚玉米粉作为生产富马酸的最佳淀粉原料。此外，对富马酸同步糖化发酵工艺进行了优化。以脱胚玉米粉为底物，在总糖浓度为100 g/L的条件下，在7 L发酵罐中进行SSF发酵，富马酸产量为35.51 g/L，产率为0.355 g/g，生产效率为0.493 [g/（L.h）]。
To reduce the manufacturing cost of fumaric acid production by fermentation, four kinds of raw starchy materials were investigated, cassava powder, corn powder, degermed corn powder and starch. By comparing the nitrogen contents in them, carbon/nitrogen ratio of raw materials was the key factor affecting the fumaric acid production by Rhizopus oryzae. Based on this point, degermed corn powder was selected as the best raw starchy material for fumaric acid production. In addition, the simultaneous saccharification and fermentation (SSF) process for fumaric acid production was optimized. With degermed corn powder as substrate at 100 g/L total sugar concentration, the fumaric acid production (35.51 g/L), yield (0.355 g/g) and productivity (0.493 [g/(L.h)]) were obtained via SSF process in a 7-L fermentor.