Inhibition of Rho-kinase induces alphaB-crystallin expression in lens epithelial cells.
Inhibition of Rho-kinase induces alphaB-crystallin expression in lens epithelial cells.
复制标题
抑制 Rho 激酶可诱导晶状体上皮细胞中 αB-晶状体蛋白的表达。
DOI：
10.1016/s0006-291x(02)00583-1
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
VasanthaRao,P
中科院分区：
文献类型：
--
作者：
Khurana,RahulN;Maddala,RupaLatha;Shimokawa,Hiroaki;SamuelZigler,J;Epstein,DavidL;VasanthaRao,P
The small heat shock protein, αB-crystallin, has been shown to interact with actin and intermediate filament proteins. However, little is known regarding the cellular mechanisms regulating such interactions. In this study, we explored the role of the Rho/Rho-kinase pathway in αB-crystallin distribution and expression in porcine lens epithelial cells. αB-crystallin was distributed uniformly throughout the cytoplasm and did not exhibit any unique redistribution in response to actin depolymerization induced by Rho/Rho-kinase inhibitors (C3-exoenzyme or Y-27632) or by overexpression of the dominant negative mutant of Rho-kinase (DNRK) in porcine lens epithelial cells. Interestingly, αB-crystallin levels markedly increased in lens epithelial cells treated with the inhibitors of Rho/Rho-kinase proteins (lovastatin, Y-27632 or DNRK) while a protein kinase C inhibitor (GF109203x) was found to have no effect. Further, Y-27632 showed a dose (2–50 μ M) response effect on αB-crystallin induction. Nocodazole, a microtubule-depolymerizing agent, elicited an increase in αB-crystallin levels but latrunculin, an actin depolymerizing agent, did not show any significant effect. Pretreatment with cycloheximide or genistein blocked the Rho-kinase inhibitor-induced increase in αB-crystallin protein levels. Rho-kinase inhibitor-induced increases in αB-crystallin levels were found to be associated with activation of P38 mitogen-activated protein kinase (MAPK). These results suggest that Rho/Rho-kinase negatively regulates αB-crystallin expression, and this response appears to be dependent on tyrosine–protein kinase and P38 MAPK function. Finally, αB-crystallin induction appears to be better correlated with the direct inhibition of Rho/Rho-kinase than with actin depolymerization per se.
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影响因子：
56.9
作者：
XIA, ZG;DICKENS, M;GREENBERG, ME
通讯作者：
GREENBERG, ME
YUTAKA INAGUMA：“大鼠神经胶质瘤 (GA-1) 细胞中热休克对 αB 晶状体蛋白的易位和诱导。”
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--
影响因子：
56.9
作者：
Kimura, K;Ito, M;Kaibuchi, K
通讯作者：
Kaibuchi, K
影响因子：
4.8
作者：
Golestaneh, N;Fan, JG;Chepelinsky, AB
通讯作者：
Chepelinsky, AB
影响因子：
64.5
作者：
Alberts, AS;Geneste, O;Treisman, R
通讯作者：
Treisman, R