Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells
Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells
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DOI：
10.1111/j.1349-7006.2009.01305.x
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发表时间：
2009-11-01
期刊：
影响因子：
5.7
通讯作者：
Imamura, Takeshi
中科院分区：
文献类型：
--
作者：
Hanyu, Aki;Kojima, Kiyotsugu;Imamura, Takeshi
血管生成在癌症进展和转移中发挥着至关重要的作用。因此，阻断肿瘤血管生成可能是预防肿瘤建立和转移的通用方法。在这项研究中，我们使用在体内和离体荧光成像显示，抗人血管内皮生长因子（VEGF）抗体抑制血管生成和移植裸鼠的人纤维肉瘤HT1080细胞的原发性肿瘤的生长。有趣的是，施用抗人VEGF抗体减少了HT1080细胞肿瘤中新血管的发育并使预先存在的肿瘤脉管系统正常化。此外，抗人VEGF抗体治疗减少了原发性肿瘤的肺转移，而在将肿瘤细胞注射到尾静脉中的肺定殖实验中，它未能阻断肺转移。这些结果表明，由原发性HT 1080细胞肿瘤产生的VEGF对肺转移具有至关重要的作用。本研究表明，在体内荧光显微镜系统将是有用的，以研究血管生成的生物学和测试的有效性，血管生成抑制剂。（Cancer Sci 2009）。
Angiogenesis plays a crucial role in cancer progression and metastasis. Thus, blocking tumor angiogenesis is potentially a universal approach to prevent tumor establishment and metastasis. In this study, we used in vivo and ex vivo fluorescence imaging to show that an antihuman vascular endothelial growth factor (VEGF) antibody represses angiogenesis and the growth of primary tumors of human fibrosarcoma HT1080 cells in implanted nude mice. Interestingly, administering the antihuman VEGF antibody reduced the development of new blood vessels and normalized pre-existing tumor vasculature in HT1080 cell tumors. In addition, antihuman VEGF antibody treatment decreased lung metastasis from the primary tumor, whereas it failed to block lung metastasis in a lung colonization experiment in which tumor cells were injected into the tail vein. These results suggest that VEGF produced by primary HT1080 cell tumors has a crucial effect on lung metastasis. The present study indicates that the in vivo fluorescent microscopy system will be useful to investigate the biology of angiogenesis and test the effectiveness of angiogenesis inhibitors. (Cancer Sci 2009).