Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs
Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs
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DOI：
10.1016/s0003-4975(02)03952-8
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发表时间：
2002-11-01
影响因子：
4.6
通讯作者：
Xiao, YF
中科院分区：
文献类型：
--
作者：
Min, JY;Sullivan, MF;Xiao, YF
背景。心肌梗死（MI）后的存活心肌细胞由于再生能力有限而无法修复坏死的心肌。本研究调查了人间充质干细胞 (hMSC) 的心肌内移植或 hMSC 与人胎儿心肌细胞 (hFC; 1:1) 的共移植是否可以重建 MI 猪受损的心肌并改善心功能。方法和结果。培养的 hMSC 用绿色荧光蛋白 (GFP) 转染。 MI 诱导和细胞移植六周后，仅移植 hMSC 的 MI 猪的心脏功能显着改善。然而，在同时移植 hMSC 和 hFC 的 MI 猪中，这种改善甚至更为显着。组织学检查表明，单独移植 hMSC 或 hMSC 加 hFC 移植可在梗塞心肌中形成 GFP 阳性移植物。此外，心脏α-肌球蛋白重链和肌钙蛋白I的免疫染色显示，在单独移植hMSC或hMSC加hFC的梗塞区域中呈阳性染色。结论。我们的数据表明，单独移植 hMSC 可以改善 MI 猪的心脏功能，而同时移植 hMSC 和 hFC 的改善效果明显更大。这种改善可能是由于移植细胞在受损心脏中的心肌再生和血管生成所致。 (C) 2002 年，胸外科医师协会。
Background. Viable cardiomyocytes after myocardial infarction (MI) are unable to repair the necrotic myocardium due to their limited capability of regeneration. The present study investigated whether intramyocardial transplantation of human mesenchymal stem cells (hMSCs) or cotransplantation of hMSCs plus human fetal cardiomyocytes (hFCs; 1:1) reconstituted impaired myocardium and improved cardiac function in MI pigs.Methods and Results. Cultured hMSCs were transfected with green fluorescent protein (GFP). Six weeks after MI induction and cell transplantation, cardiac function was significantly improved in MI pigs transplanted with hMSCs alone. However, the improvement was even markedly greater in MI pigs cotransplanted with hMSCs plus hFCs. Histological examination demonstrated that transplantation of hMSCs alone or hMSCs plus hFCs formed GFP-positive engrafts in infarcted myocardium. In addition, immunostaining for cardiac a-myosin heavy chain and troponin I showed positive stains in infarcted regions transplanted with hMSCs alone or hMSCs plus hFCs.Conclusions. Our data demonstrate that transplantation of hMSCs alone improved cardiac function in MI pigs with a markedly greater improvement from cotransplantation of hMSCs plus hFCs. This improvement might result from myocardial regeneration and angiogenesis in injured hearts by engrafted cells. (C) 2002 by The Society of Thoracic Surgeons.