Practical method for optimizing radioimmunoassay detection and precision limits.
Practical method for optimizing radioimmunoassay detection and precision limits.
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优化放射免疫分析检测和精度限值的实用方法。
DOI：
10.1093/clinchem/37.2.226
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发表时间：
1991
影响因子：
9.3
通讯作者：
F. Dray
中科院分区：
文献类型：
--
作者：
E. Ezan;C. Tiberghien;F. Dray
基于质量作用定律的竞争性放射免疫测定标准曲线模型已经开发出来，并与实验和计数误差结合使用，以预测测定检测限和精度曲线。我们通过实验室进行的半抗原放射免疫测定验证了该模型。由此产生的计算机程序可用于确定最佳抗血清浓度（取决于其亲和力）和标记抗原浓度（根据其特异性活性和非特异性结合）。该模型的图形表示为放射免疫学家提供了优化测定的实用工具。
A model of the competitive radioimmunoassay standard curve, based on the Law of Mass Action, has been developed and used in conjunction with experimental and counting errors to predict the assay detection limit and precision profiles. We verified the model with hapten radioimmunoassays performed in our laboratory. The resulting computer program can be used to determine the optimum antiserum concentration--depending on its affinity--and labeled-antigen concentration--according to its specific activity and nonspecific binding. The graphical representation of this model provides radioimmunologists with a practical tool for assay optimization.