The human PIN1 peptidyl-prolyl cis/trans isomerase gene maps to human chromosome 19p13 and the closely related PIN1L gene to 1p31
The human PIN1 peptidyl-prolyl cis/trans isomerase gene maps to human chromosome 19p13 and the closely related PIN1L gene to 1p31
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DOI：
10.1006/geno.1997.4854
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发表时间：
1997-09-01
期刊：
影响因子：
4.4
通讯作者：
Young, IG
中科院分区：
文献类型：
--
作者：
Campbell, HD;Webb, GC;Young, IG
人PIN 1基因编码一种参与有丝分裂调控的必需核肽基脯氨酰顺/反异构酶。PIN 1是一类新的肽基脯氨酰顺/反异构酶，包括大肠杆菌小蛋白，酵母ESS 1，和果蝇dodo基因产物的成员，人类EST的分析表明，有两个不同的，但密切相关的人类转录本，其中之一对应于PIN 1。使用FISH和氚标记探针的基因定位显示，每个人类转录本与1 p31和19 p13杂交。设计引物以区分两种转录本，并使用保留1号或19号染色体的仓鼠/人类体细胞杂交体的DNA进行PCR，以将人类PIN 1基因定位于19号染色体，并将密切相关的基因PIN 1 L定位于1号染色体。结果表明，PIN 1位于19 p13，PIN 1 L位于1 p31，用PCR方法克隆了PIN 1 L的编码区。PIN 1 L cDNA在核苷酸水平上与PIN 1转录物具有89%的同一性，但在阅读框中含有移位。它编码一个100个氨基酸的变体蛋白，由63个与PIN 1同源（90%相同）的氨基酸组成，并含有整个WW结构域，融合到一个37个氨基酸的尾部。由PIN 1 L编码的蛋白质可能具有某些功能作用，或者PIN 1 L可能是转录的假基因。(C)北京：科学出版社.
The human PIN1 gene encodes an essential nuclear peptidyl-prolyl cis/trans isomerase involved in the regulation of mitosis. PIN1 is a member of a new class of peptidyl-prolyl cis/trans isomerases that includes the Escherichia coli parvulin, yeast ESS1, and Drosophila melanogaster dodo gene products, Analysis of human ESTs showed that there are two different but closely related human transcripts, one of which corresponds to PIN1. Gene localization, using both FISH and tritium-labeled probes, showed that each of the human transcripts hybridized to 1p31 and 19p13. Primers were designed to discriminate between the two transcripts, and PCR on DNA from hamster/human somatic cell hybrids retaining chromosomes 1 or 19 was used to map the human PIN1 gene to chromosome 19, and PIN1L, a closely related gene, to chromosome 1. The results establish that PIN1 is at 19p13 and PIN1L at 1p31, PCR was used to clone the coding region for PIN1L. The PIN1L cDNA is 89% identical at the nucleotide level to the PIN1 transcript, but contains a shift in the reading frame. It encodes a 100-amino-acid variant protein consisting of 63 amino acids homologous (90% identical) to PIN1 and containing the entire WW domain, fused to a 37-amino-acid tail. The protein encoded by PIN1L may have some functional role or alternatively PIN1L may be a transcribed pseudogene. (C) 1997 Academic Press.