Accurate targeting of activated macrophages based on synergistic activation of functional molecules uptake by scavenger receptor and matrix metalloproteinase.
Accurate targeting of activated macrophages based on synergistic activation of functional molecules uptake by scavenger receptor and matrix metalloproteinase.
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DOI：
10.1021/cb800067e
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发表时间：
2008-07
影响因子：
4
通讯作者：
Hideyuki Suzuki;Moritoshi Sato;Y. Umezawa
中科院分区：
文献类型：
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作者：
Hideyuki Suzuki;Moritoshi Sato;Y. Umezawa
特异性靶向疾病细胞具有深远的应用潜力，例如疾病的诊断成像和治疗。在这里，我们描述了一种在各种细胞类型中选择性靶向一种细胞的新方法。活化的巨噬细胞是与动脉粥样硬化的发生和发展相关的疾病细胞。我们开发了一种分子探针，其中摄取到细胞协同激活清道夫受体A类I型（SR-AI）和基质金属蛋白酶-9（MMP-9），这是标志性受体和标志性蛋白酶的动脉粥样硬化，分别。我们证明了本发明的靶向探针选择性地掺入表达SR-AI和MMP-9的活化形式两者的活化巨噬细胞中，但不掺入仅具有SR-AI的静息巨噬细胞中。本方法可以为细胞特异性成像和治疗提供有力的工具。
Specific targeting of disease cells has the potential of far-reaching applications, such as diagnostic imaging and therapies of diseases. Here we describe a novel method for selective targeting of a type of cells among various cell types. Activated macrophages are disease cells related to initiation and progression of atherosclerosis. We developed a molecular probe of which uptake into cells is synergistically activated by scavenger receptor class A type I (SR-AI) and matrix metalloproteinase-9 (MMP-9), which are the marker receptor and the marker protease of atherosclerosis, respectively. We demonstrated that the present targeting probe is selectively incorporated into activated macrophages expressing both SR-AI and the activated form of MMP-9 but not into resting macrophages having only SR-AI. The present approach may provide a powerful tool for cell-specific imaging and therapies.