[miR-29b Reduces Cisplatin Resistance of Gastric Cancer Cell by Targeting PI3K/Akt Pathway].
[miR-29b Reduces Cisplatin Resistance of Gastric Cancer Cell by Targeting PI3K/Akt Pathway].
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DOI：
10.3881/j.issn.1000-503x.2015.05.005
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发表时间：
2015-10-01
期刊：
影响因子：
--
通讯作者：
Wang, Ya-di
中科院分区：
文献类型：
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作者：
Chen, Dian-dian;Feng, Lin-chun;Wang, Ya-di
目的：探讨miR-29b对胃细胞顺铂耐药的调控作用。方法：采用定量逆转录聚合酶链式反应（qRT-PCR）和Western blotting检测顺铂浓度梯度处理胃癌细胞株中miR-29b的表达。使用CCK8检测miR-29b敲低和过表达情况下顺铂治疗后的细胞活力。结果：顺铂治疗后miR-29b表达明显上调，其靶基因AKT2表达下调。miR-29b的上调增强了胃癌细胞对顺铂的敏感性，miR-29b的下调增强了胃癌细胞对顺铂的耐药性。救援实验证实miR-29b可能通过靶向PI3K/Akt通路调控胃癌细胞的顺铂耐药。miR-29家族的另外两个成员miR-29a/c的表达在顺铂治疗后得到促进，但对胃癌细胞对顺铂的耐药无显著影响。结论：miR-29b可通过直接靶向PI3K/Akt通路增强S胃癌细胞的敏感性。
OBJECTIVE: To investigate the regulatory effect of miR-29b on gastric cells' resistance to cisplatin.METHODS: The expression of miR-29b in gastric cancer cell line treated with cisplatin concentration gradient was detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. CCK8 was used to measure the cell viability after cisplatin treatment in condition of miR-29b knock-down and overexpression.RESULTS: The expression of miR-29b was significantly upregualted by cisplatin treatment,while its target gene AKT2 was downregulated. The up-regulation of miR-29b enhanced the sensitivity of gastric cancer cells to cisplatin,while the knock-down of miR-29b enhanced the cisplatin resistance. Rescue experiments demonstrated that the miR-29b might regulate cisplatin resistance of gastric cancer cell by targeting PI3K/Akt pathway. The expressions of the other two members of miR-29 family, miR-29a/c, were promoted by cisplatin treatment,but they had no significant effect on gastric cancer cell's resistance to cisplatin.CONCLUSION: miR-29b can enhance the sensitivity of S gastric cancer cell by directly targeting PI3K/Akt pathway.