MRI can reveal metabolic changes in lily bulbs in vivo during dormancy release
MRI can reveal metabolic changes in lily bulbs in vivo during dormancy release
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DOI：
10.1007/s11427-012-4394-8
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发表时间：
2012-10
期刊：
影响因子：
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通讯作者：
Hao-jun Han;M. Yi
中科院分区：
文献类型：
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作者：
Hao-jun Han;M. Yi
影响百合鳞茎休眠解除的因素对商品化的成功与否有着重要影响，但其休眠解除的机理尚不清楚。磁共振成像（MRI）可以检测到形态和水状态的变化，在一个活的植物鳞茎，并协助调查释放因子。为了评估MRI是否可用于检测东方百合（Lilium'Sorbonne'）休眠期内鳞茎内代谢变化，每周对在4°C下储存11周的鳞茎进行一系列MRI和糖浓度测量。球内结构T1加权成像的图像质量上级T2加权成像，信噪比（0.97±0.01）高。在冷藏的前8周内，花蕾和基盘的磁化传递率值均呈下降趋势（P>0.05），且与花蕾（R2=0.95）和基盘（R2=0.93）的可溶性糖浓度呈良好的相关性。因此，MRI可以作为一个有价值的工具，观察和分析的动态形态和代谢变化在体内休眠解除。这一信息是潜在的有用的指导，在园艺产品质量的提高。
The factors influencing dormancy release in lily bulbs strongly affect commercialization success, but the mechanism of dormancy release is still unclear. Magnetic resonance imaging (MRI) can detect changes in morphology and water status in a living plant bulb and aid in investigating release factors. To evaluate whether MRI could be used to detect intra-bulb metabolic changes during the dormant period in Oriental Lilies (Lilium‘Sorbonne’), a series of MRI and sugar concentration measurements were performed weekly on bulbs stored for 11 weeks at 4°C. The image quality of intra-bulb structure obtained usingT1-weighted imaging was superior to that obtained usingT2-weighted imaging and had a higher signal-to-noise ratio (0.97±0.01). Magnetization transfer ratio values for the bud and basal plate declined during the first eight weeks of cold storage (P>0.05), and were well correlated with concentration of soluble sugar in the bud (R2=0.95) and basal plate (R2=0.93). Thus, MRI can serve as a valuable tool for observation and analysis of dynamic morphological and metabolic changesin vivoduring dormancy release. This information is potentially useful as a guide in the improvement of horticultural product quality.