Evaluation of acute corneal damage induced by 222-nm and 254-nm ultraviolet light in Sprague-Dawley rats
Evaluation of acute corneal damage induced by 222-nm and 254-nm ultraviolet light in Sprague-Dawley rats
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DOI：
10.1080/10715762.2019.1603378
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发表时间：
2019-06-03
影响因子：
3.3
通讯作者：
Tanito, Masaki
中科院分区：
文献类型：
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作者：
Kaidzu, Sachiko;Sugihara, Kazunobu;Tanito, Masaki
氪氯准分子灯产生的222纳米紫外线对细菌细胞有害，但对皮肤无害。然而，222纳米紫外线对眼睛的影响还不完全清楚。我们评估了222 nm和254 nm紫外线对白化大鼠的急性角膜损伤。在深度麻醉下，6周龄SD大鼠接受紫外线照射。角膜照射水平分别为30、150和600mJ/cm(2)。用荧光素染色检测上皮细胞的缺陷。在超过150mJ/cm(2)的紫外线照射下，角膜出现浅表性点状角膜炎，在600mJ/cm(2)的紫外线照射下，角膜出现侵蚀。苏木精-伊红染色还显示了暴露在254 nm紫外光下的眼睛的角膜上皮缺陷。然而，暴露在222纳米紫外光下的角膜没有出现损伤。环丁烷嘧啶二聚体阳性细胞仅见于正常角膜和经254 nm紫外光照射的角膜。正常角膜虽然部分上皮细胞染色较弱，但鳞状上皮细胞呈中度染色，暴露于600mJ/cm(2)光照下的角膜上皮层在254 nm紫外光照射下呈深染。在目前的研究中，222 nm紫外光没有引起角膜损伤，这表明222 nm紫外光可能在能量范围内不会损害大鼠的眼睛，并可能在未来用于消毒或预防感染。
Two hundred twenty-two nanometres ultraviolet (UV) light produced by a krypton-chlorine excimer lamp is harmful to bacterial cells but not skin. However, the effects of 222-nm UV light exposure to the eye are not fully known. We evaluated acute corneal damage induced by 222- and 254-nm UV light in albino rats. Under deep anaesthesia, 6-week-old Sprague-Dawley albino rats were exposed to UV light. The exposure levels of corneal radiation were 30, 150, and 600mJ/cm(2). Epithelial defects were detected by staining with fluorescein. Superficial punctate keratitis developed in corneas exposed to more than 150mJ/cm(2) of UV light, and erosion was observed in corneas exposed to 600mJ/cm(2) of UV light. Haematoxylin and eosin staining also showed corneal epithelial defects in eyes exposed to 254-nm UV light. However, no damage developed in corneas exposed to 222-nm UV light. Cyclobutane pyrimidine dimer-positive cells were observed only in normal corneas and those exposed to 254-nm UV light. Although some epithelial cells were stained weakly in normal corneas, squamous epithelial cells were stained moderately, and the epithelial layer that was detached from the cornea exposed to 600mJ/cm(2) of light was stained intensely in corneas exposed to 254-nm UV light. In the current study, no corneal damage was induced by 222-nm UV light, which suggested that 222-nm UV light may not harm rat eyes within the energy range and may be useful for sterilising or preventing infection in the future.