Normal colonic epithelium adheres to carcinoembryonic antigen and type IV collagen.
Normal colonic epithelium adheres to carcinoembryonic antigen and type IV collagen.
复制标题
正常结肠上皮粘附癌胚抗原和IV型胶原。
DOI：
10.1016/0016-5085(94)90015-9
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发表时间：
1994
期刊：
影响因子：
29.4
通讯作者：
Jessup,JM
中科院分区：
文献类型：
--
作者：
Ishii,S;SteeleJr,G;Ford,R;Paliotti,G;Thomas,P;Andrews,C;Hansen,HJ;Goldenberg,DM;Jessup,JM
背景/目的：人结直肠癌细胞与基底膜中的胶原和层粘连蛋白以及邻近细胞上的癌胚抗原（CEA）结合。本研究的目的是确定是否正常的结肠上皮细胞结合CEA，胶原蛋白，或lamininin.Methods：完整的结肠隐窝中分离出13个标本切除结直肠癌或结肠憩室炎的正常粘膜。结肠细胞被释放的隐窝通过与胶原酶和脱氧核糖核酸酶的治疗和测试粘附CEA，IV型胶原，和层粘连蛋白在固相adhesionassay.Results：12%至25%的结肠细胞在所有标本结合到CEA。从7个标本的结肠细胞也绑定到IV型胶原蛋白，但没有一个结肠细胞制剂绑定显着层粘连蛋白。CEA和透明质酸受体CD 44的单克隆抗体和膜CEA的酶促去除阻断了结肠细胞对CEA.Conclusions的粘附：第一，结肠细胞使用相同的表位上的CEA和CD 44作为结直肠癌细胞粘附到固相CEA。第二，结肠细胞通过CEA对CEA的嗜同性结合与固相CEA结合。第三，CEA和IV型胶原蛋白，而不是层粘连蛋白，是人类结肠细胞的粘附配体。
Background/Aims:Human colorectal carcinoma cells bind to collagen and laminin in the basement membrane as well as to carcinoembryonic antigen (CEA) on neighboring cells. The purpose of this study was to determine whether normal colonie epithelial cells bind to CEA, collagen, or laminin.Methods:Intact colonic crypts were isolated from normal mucosa in 13 specimens resected for colorectal carcinoma or colonic diverticulitis. Colonocytes were released from the crypts by treatment with collagenase and deoxyribonuclease and tested for adhesion to CEA, type IV collagen, and laminin in a solid-phase adhesion assay.Results:Twelve percent to 25% of colonocytes in all specimens bound to CEA. Colonocytes from seven specimens also bound to type IV collagen, but none of the colonocyte preparations bound significantly to laminin. Monoclonal antibodies to CEA and to the hyaluronate receptor CD44 and enzymatic removal of membrane CEA blocked the adhesion of colonocytes to CEA.Conclusions:First, colonocytes use the same epitopes on CEA and CD44 as colorectal carcinoma cells to adhere to solid-phase CEA. Second, colonocytes bind to solid-phase CEA through CEA-to-CEA homophilic binding. Third, CEA and type IV collagen, but not laminin, are adhesion ligands for human colonocytes.