Transcription-dependent spatial arrangements of CFTR and adjacent genes in human cell nuclei
Transcription-dependent spatial arrangements of CFTR and adjacent genes in human cell nuclei
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人类细胞核中 CFTR 和邻近基因的转录依赖性空间排列
DOI：
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发表时间：
2004
影响因子：
7.8
通讯作者：
D. Schindelhauer
中科院分区：
文献类型：
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作者：
D. Zink;M. Amaral;Andreas Englmann;Susanne B Lang;L. Clarke;C. Rudolph;F. Alt;K. Luther;C. Braz;Nicolas Sadoni;J. Rosenecker;D. Schindelhauer
我们在不同的人类细胞类型的核定位和转录调控的功能无关的基因GASZ，CFTR和CORTBP 2，映射到人类染色体7q31上的相邻位点。当不活跃时，GASZ、CFTR和CORTBP 2优先与核外围和核周异染色质相关，而在其活跃转录状态下，基因位点优先与核内部的常染色质相关。相邻基因同时与这些不同的染色质组分定位在不同的核区域，根据其各自的转录调控。虽然CFTR的核定位改变后，改变其转录水平，CFTR的转录状态并没有改变驱动该基因进入不同的核环境。这意味着转录活性影响核定位，而不是相反。总之，这些结果表明，小的染色体亚区可以显示高度灵活的核组织，这些组织以转录依赖的方式在单个基因水平上受到调控。
We investigated in different human cell types nuclear positioning and transcriptional regulation of the functionally unrelated genes GASZ, CFTR, and CORTBP2, mapping to adjacent loci on human chromosome 7q31. When inactive, GASZ, CFTR, and CORTBP2 preferentially associated with the nuclear periphery and with perinuclear heterochromatin, whereas in their actively transcribed states the gene loci preferentially associated with euchromatin in the nuclear interior. Adjacent genes associated simultaneously with these distinct chromatin fractions localizing at different nuclear regions, in accordance with their individual transcriptional regulation. Although the nuclear localization of CFTR changed after altering its transcription levels, the transcriptional status of CFTR was not changed by driving this gene into a different nuclear environment. This implied that the transcriptional activity affected the nuclear positioning, and not vice versa. Together, the results show that small chromosomal subregions can display highly flexible nuclear organizations that are regulated at the level of individual genes in a transcription-dependent manner.
影响因子：
--
作者：
Wei Yan;A. Rajkovic;M. Viveiros;K. Burns;J. Eppig;M. Matzuk
通讯作者：
Wei Yan;A. Rajkovic;M. Viveiros;K. Burns;J. Eppig;M. Matzuk
影响因子：
15.9
作者：
ENGELHARDT, JF;ZEPEDA, M;WILSON, JM
通讯作者：
WILSON, JM
影响因子：
10.5
作者：
Ayyanathan, K;Lechner, MS;Rauscher, FJ
通讯作者：
Rauscher, FJ