A fluorometric method for the differentiation of algal populations in vivo and in situ
A fluorometric method for the differentiation of algal populations in vivo and in situ
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DOI：
10.1023/a:1016026607048
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发表时间：
2002-01-01
影响因子：
3.7
通讯作者：
Dau, H
中科院分区：
文献类型：
--
作者：
Beutler, M;Wiltshire, KH;Dau, H
叶绿素荧光激发光谱的指纹可用于区分体内和原位微藻的“光谱组”，例如，几秒钟内的垂直分布。调查光谱组的藻类（绿色组，绿藻;蓝色，蓝细菌;棕色，Heterokontophyta，Haptophyta，Dinophyta;混合，Cryptophyta）的每个特征是由一个特定的组合物的光合天线色素，因此，由一个特定的激发光谱的叶绿素荧光。特别相关的是Chla、Chlc、藻蓝胆素、藻红胆素、岩藻黄素和多甲藻黄素。一个实验室为基础的仪器和潜水仪器的构造包含发光二极管激发叶绿素荧光在五个不同的波长范围。标准光谱测定的四个光谱藻类组（每组几个物种）。使用这些规范的光谱和实际的五点激发光谱的水样，一个单独的估计各自的叶绿素浓度迅速获得每个藻类组。给出了稀释实验的结果。在体内和原位测量进行比较，通过HPLC分析得到的结果。显示了在几秒钟的时间内拍摄的光谱藻类群分布的深度剖面。这里描述的藻类分化的方法开辟了新的研究领域，监测和监督任务有关的光合初级生产在水生环境中。
Fingerprints of excitation spectra of chlorophyll (Chl) fluorescence can be used to differentiate `spectral groups' of microalgae in vivo and in situ in, for example, vertical profiles within a few seconds. The investigated spectral groups of algae (green group, Chlorophyta; blue, Cyanobacteria; brown, Heterokontophyta, Haptophyta, Dinophyta; mixed, Cryptophyta) are each characterised by a specific composition of photosynthetic antenna pigments and, consequently, by a specific excitation spectrum of the Chl fluorescence. Particularly relevant are Chl a, Chl c, phycocyanobilin, phycoerythrobilin, fucoxanthin and peridinin. A laboratory-based instrument and a submersible instrument were constructed containing light-emitting diodes to excite Chl fluorescence in five distinct wavelength ranges. Norm spectra were determined for the four spectral algal groups (several species per group). Using these norm spectra and the actual five-point excitation spectrum of a water sample, a separate estimate of the respective Chl concentration is rapidly obtained for each algal group. The results of dilution experiments are presented. In vivo and in situ measurements are compared with results obtained by HPLC analysis. Depth profiles of the distribution of spectral algal groups taken over a time period of few seconds are shown. The method for algae differentiation described here opens up new research areas, monitoring and supervision tasks related to photosynthetic primary production in aquatic environments.