TISSUE SPECIFIC EXPRESSION OF P422 PROTEIN, A PUTATIVE LIPID CARRIER, IN MOUSE ADIPOCYTES
TISSUE SPECIFIC EXPRESSION OF P422 PROTEIN, A PUTATIVE LIPID CARRIER, IN MOUSE ADIPOCYTES
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DOI：
10.1016/0006-291x(85)91209-4
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
LANE, MD
中科院分区：
文献类型：
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作者：
BERNLOHR, DA;DOERING, TL;LANE, MD
3 T3-L1前脂肪细胞的分化导致新蛋白p422及其mRNA的表达。该蛋白质分别与肝和肠的髓鞘P2和脂肪酸结合蛋白具有70%和20-30%的氨基酸序列同源性。通过免疫印迹法和cDNA杂交法对p422蛋白在小鼠组织中的分布和p422 mRNA在小鼠组织中的分布的研究表明，它们仅在脂肪组织中表达。在小鼠脂肪组织或3 T3-L1脂肪细胞中未检测到肝和肠脂肪酸结合蛋白mRNA。提示p422是脂肪细胞脂肪酸结合蛋白。
The differentiation of 3T3-L1 preadipocytes leads to the expression of a new protein, p422, and its mRNA. This protein has 70% and 20-30% amino acid sequence homology to myelin P2 and the fatty acid binding proteins of liver and intestine, respectively. Investigation of the distribution in mouse tissues of p422 protein by immunoblotting and of p422 mRNA by cDNA hybridization indicates that they are expressed only in adipose tissue. Liver and intestinal fatty acid binding protein mRNA''s were not detectable in mouse adipose tissue or in 3T3-L1 adipocytes. It is suggested that p422 functions as an adipocyte fatty acid binding protein.