Immunolocalization of vascular endothelial growth factor during heterotopic bone formation induced from grafted periosteum
Immunolocalization of vascular endothelial growth factor during heterotopic bone formation induced from grafted periosteum
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DOI：
10.1097/01.sap.0000110752.55981.41
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发表时间：
2004-08-01
影响因子：
1.5
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Ueno, T;Kagawa, T;Yamamoto, T
血管侵袭是软骨替代导致骨形成的重要步骤，并且血管内皮生长因子（VEGF）已被认为是该过程中的关键参与者。虽然移植骨膜会发生软骨内骨化，但对VEGF在此过程中的作用知之甚少。在目前的研究中，作者通过免疫组织化学、组织化学和超微结构技术研究了骨膜移植骨形成过程中VEGF的定位。日本白色兔胫骨移植后第14天，移植组织中的骨膜细胞已分化为软骨细胞，形成软骨。一些软骨细胞VEGF表达呈免疫阳性，随后的血管浸润主要发生在这些VEGF阳性区域。在第45天，被血管侵入的软骨已被新形成的骨所取代。这些结果表明，VEGF与血管侵入软骨的过程中，骨置换之前，从移植骨膜软骨内骨化。
Vessel invasion is an important step in cartilage replacement that leads to bone formation, and vascular endothelial growth factor (VEGF) has been implicated as a key player in this process. Although grafted periosteum undergoes endochondral ossification, little is known about the role of VEGF in this process. In the current study the authors investigated by immunohistochemical, histochemical, and ultrastructural techniques the localization of VEGF during bone formation in periosteal grafts. At day 14 after grafting the tibias of Japanese white rabbits, periosteal cells in the grafted tissue had differentiated into chondrocytes to form cartilage. Some chondrocytes were immunopositive for VEGF expression, and subsequent vessel invasion occurred predominantly in these VEGF-positive areas. At day 45, the cartilage invaded by blood vessels had been replaced by newly formed bone. These findings suggest that VEGF is associated with the process of blood vessel invasion into cartilage before bone replacement in endochondral ossification from grafted periosteum.