Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery Tools Can Contribute to Precise and Quantitative Cell Biology Assays.
Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery Tools Can Contribute to Precise and Quantitative Cell Biology Assays.
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高效、无害的活细胞递送：使膜屏障消失以实现细胞生物化学：更好的细胞递送工具如何有助于精确和定量的细胞生物学测定。
DOI：
10.1002/bies.201900031
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Pellois,Jean-Philippe
中科院分区：
文献类型：
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作者：
Pellois,Jean-Philippe
蛋白质工程技术和递送方案的融合为细胞生物学提供了新的机会。特别是，使细胞膜瞬时渗透的技术使将非遗传编码的大分子探针引入细胞成为可能。这反过来又能够以既精确又定量的方式监测细胞内的过程，开创了一个可以想象为细胞生物化学的领域。在这里，作者回顾了这种基于细胞的新分析的开创性例子，提供了挑战细胞渗透对细胞必然是破坏性和应激性事件这一范式的证据，并强调了为充分释放这一新兴领域的潜力而应解决的一些挑战。
The confluence of protein engineering techniques and delivery protocols are providing new opportunities in cell biology. In particular, techniques that render the membrane of cells transiently permeable make the introduction of nongenetically encodable macromolecular probes into cells possible. This, in turn, can enable the monitoring of intracellular processes in ways that can be both precise and quantitative, ushering an area that one may envision as cellular biochemistry. Herein, the author reviews pioneering examples of such new cell‐based assays, provides evidence that challenges the paradigm that cell penetration is a necessarily damaging and stressful event for cells, and highlights some of the challenges that should be addressed to fully unlock the potential of this nascent field.