Suppressor analyses of temperature-sensitive cbp1 strains of Saccharomyces cerevisiae: the product of the nuclear gene SOC1 affects mitochondrial cytochrome b mRNA post-transcriptionally.
Suppressor analyses of temperature-sensitive cbp1 strains of Saccharomyces cerevisiae: the product of the nuclear gene SOC1 affects mitochondrial cytochrome b mRNA post-transcriptionally.
复制标题
酿酒酵母温度敏感 cbp1 菌株的抑制子分析：核基因 SOC1 的产物在转录后影响线粒体细胞色素 b mRNA。
DOI：
10.1093/genetics/138.3.565
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Dieckmann,CL
中科院分区：
文献类型：
--
作者：
Staples,RR;Dieckmann,CL
线粒体功能的诱导依赖于核编码和线粒体编码的基因产物。要了解核线粒体相互作用，我们必须首先了解基因特异性相互作用。线粒体细胞色素 b (COB) RNA 的积累依赖于核基因 CBP1 编码的 Cbp1p。因此，呼吸依赖于 Cbp1p。在这项研究中，选择了温度敏感的 cbp1 (cbp1ts) 菌株的抑制剂来恢复限制温度 Ts+ 下的呼吸能力。一种核编码的抑制基因，是 CBP1 的外源基因，相对于野生型抑制基因等位基因是隐性的，并且与其他已知的基因位点无关，而其他已知的基因位点的基因产物是 COB mRNA 表达所必需的。该抑制子称为 soc1（cbp1 的抑制子），可抑制其他几个 cbp1ts 等位基因，但不通过旁路机制发挥作用。分子分析表明，soc1 允许 COB mRNA 的稳态水平在高温下增加，但对 COB 前体 mRNA 的水平影响很小或没有影响。这些数据使我们提出，核基因 SOC1 的产物是 COB mRNA 正常周转所必需的。
The induction of mitochondrial function is dependent upon both nuclearly encoded and mitochondrially encoded gene products. To understand nuclear-mitochondrial interactions, we must first understand gene-specific interactions. The accumulation of mitochondrial cytochrome b (COB) RNA is dependent upon Cbp1p, encoded by the nuclear gene CBP1. Thus, respiration is dependent upon Cbp1p. In this study, suppressors of temperature-sensitive cbp1 (cbp1ts) strains were selected for restoration of respiratory capability at the restrictive temperature Ts+). One nuclearly encoded suppressor, extragenic to CBP1, is recessive with respect to the wild-type suppressor allele and is unlinked to other known genetic loci whose gene products are necessary for expression of COB mRNA. The suppressor, called soc1 for Suppressor of cbp1, suppresses several other cbp1ts alleles but does not operate via a bypass mechanism. Molecular analyses indicate that soc1 allows the steady-state level of COB mRNA to increase at high temperature but has little or no effect on the levels of COB pre-mRNA. These data have led us to propose that the product of the nuclear gene SOC1 is required for normal turnover of COB mRNA.