Targeted disruption of the blood-brain barrier with focused ultrasound: association with cavitation activity
Targeted disruption of the blood-brain barrier with focused ultrasound: association with cavitation activity
复制标题
DOI：
10.1088/0031-9155/51/4/003
复制
发表时间：
2006-02-21
影响因子：
3.5
通讯作者：
Hynynen, K
中科院分区：
文献类型：
--
作者：
McDannold, N;Vykhodtseva, N;Hynynen, K
在聚焦超声暴露期间，结合超声造影剂(Optison((R)监测声发射，以确定空化活动是否与血脑屏障破坏(BBBD)的诱导有关。在兔脑内10 mm深的靶点(N=9)进行34个部位的超声刺激(频率：260 kHz)。超声峰值为0.11~0.57 Mpa(爆发长度：10ms；重复频率：1 Hz；持续时间：20 S)。用I聚焦式被动空化探测器记录声发射。在使用和不使用Optison((注册商标))的情况下，在每个位置都记录了这种发射。仅在0.40和0.57兆帕下观察到可检测到的宽带声发射。在0.29~0.57 Mpa的超声压力下，对比MR1观察到BBBD。小范围外渗红细胞的出现似乎与这一宽带发射信号有关。因此，结果表明，在Optison(R)存在的情况下，聚焦超声脉冲导致的BBBD可以在没有体内惯性空化、宽带发射和渗出的指标的情况下发生。如果惯性空化不是导致BBBD的原因，那么其他超声/微泡相互作用可能是BBBD的根源。在超声驱动频率的第二和第三次谐波处，由于Optison((R))引起的发射信号的显著增加被发现与BBBD相关，并且可能作为一种在线方法来指示何时发生干扰。
Acoustic emission was monitored during focused ultrasound exposures in conjunction with an ultrasound contrast agent (Optison((R))) in order to determine if cavitation activity is associated with the induction of blood-brain barrier disruption (BBBD). Thirty-four locations were sonicated (frequency: 260 kHz) at targets 10 mm deep in rabbit brain (N = 9). The sonications were applied at peak pressure amplitudes ranging from 0.11 to 0.57 MPa (burst length: 10 ms; repetition frequency of 1 Hz; duration: 20 s). Acoustic emission was recorded with I focused passive cavitation detector. This emission was recorded at each location during sonications with and without Optison((R)). Detectable wideband acoustic emission was observed only at 0.40 and 0.57 MPa. BBBD was observed in contrast MR1 after sonication at 0.29-0.57 MPa. The appearance of small regions of extravasated erythrocytes appeared to be associated with this wideband emission signal. The results thus suggest that BBBD resulting from focused ultrasound pulses in the presence of Optison((R)) can occur Without indicators for inertial cavitation in vivo, wideband emission and extravasation. If inertial cavitation is not responsible for the BBBD, other ultrasound/rnicrobubble interactions are likely the Source. A significant increase in the emission signal due to Optison((R)) at the second and third harmonics of the ultrasound driving frequency was found to correlate with BBBD and might be useful as an online method to indicate when the disruption occurs.