Neural-specific expression of miR-344-3p during mouse embryonic development
Neural-specific expression of miR-344-3p during mouse embryonic development
复制标题
小鼠胚胎发育过程中 miR-344-3p 的神经特异性表达
DOI：
10.1007/s10735-013-9555-y
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发表时间：
2014-08-01
影响因子：
3.2
通讯作者：
Wu, Qiong
中科院分区：
文献类型：
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作者：
Liu, Qi;He, Hongjuan;Wu, Qiong
MicroRNAs是一种小的非编码RNA，参与各种生物过程。我们研究了miR-344-3p在小鼠胚胎发育过程中的表达。在E9.5-E10.5和E15.5，原位杂交在大脑皮质、后脑、小脑、丘脑、后脑、延髓、脊髓和背根神经节等中枢神经系统检测到较强的miR-344-3p信号。此外，qRT-PCR分析发现miR-344-3p在E15.5有表达，在E12.5到E18.5期间在大脑中稳定表达，然后在出生后下降到相对较低的水平。我们还使用免疫荧光原位杂交技术分析了miR-344-3p在E18.5和成人脑内的表达。MIR-344-3P信号主要分布在脑室系统周围皮质、脉络丛、嗅球肾小球层和小脑皮质颗粒细胞层。综上所述，我们的结果表明miR-344-3p可能在大脑的形态发生、神经系统发育中发挥重要作用。
MicroRNAs are small noncoding RNAs involved in various biological processes. We characterized the expression of miR-344-3p during mouse embryonic development. At E9.5-E10.5 and E15.5, in situ hybridization detected strong miR-344-3p signal in the central nervous system, including the cerebral cortex, hindbrain, cerebellum, thalamus, hindbrain, medulla oblongata, spinal cord, and dorsal root ganglia. Further, qRT-PCR analysis identified miR-344-3p expression at E15.5, with expression stably maintained in the brain from E12.5 to E18.5 before decreasing to relatively low levels postnatally. We also analyzed miR-344-3p expression using immunofluorescence in situ hybridization at E18.5 and within the adult brain. miR-344-3p signal was mainly detected in cortical regions surrounding the ventricular system, choroid plexus, glomerular layer of the olfactory bulb, and granular cell layer of the cerebellar cortex. Altogether, our results indicate miR-344-3p may play an important role in morphogenesis, nervous system development in the brain.