Interaction of platinum compounds with short oligodeoxynucleotides containing guanine and cytosine.
Interaction of platinum compounds with short oligodeoxynucleotides containing guanine and cytosine.
复制标题
铂化合物与含有鸟嘌呤和胞嘧啶的短寡脱氧核苷酸的相互作用。
DOI：
10.1111/j.1432-1033.1983.tb07660.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
J. Reedijk
中科院分区：
文献类型：
--
作者：
A. Marcelis;J. H. J. D. Hartog;G. A. V. Marel;Gerry Wille;J. Reedijk
用质子核磁共振谱、圆二色性谱和Pt分析等方法对cis-Pt（NH_3）_2Cl_2与d（CpCpGpG）、d（GpCpG）、d（pCpGpCpG）、d（pGpCpGpC）和d（CpGpCpG）反应的产物以及[Pt（dien）Cl]Cl与d（CpCpGpG）和d（GpCpG）反应的产物进行了表征。Pt化合物的结合位点通过pH依赖的NMR光谱测定。两种Pt化合物的结合总是发生在鸟嘌呤N7原子处。在所有含有[cis-Pt（NH3）2]2+的化合物中，螯合物是通过铂与同一寡核苷酸的两个鸟嘌呤配位而形成的。所得链内交联的寡核苷酸含有d（GpG）。顺式Pt单位，或d（GpCpG）。cisPt单位。在后一种情况下，中间胞嘧啶不与铂配位。因此，源自这两种螯合物的构象变化彼此不同。在[Pt（dien）Cl]Cl作为起始产物的情况下，形成两种类型的寡核苷酸加合物，即具有一个Pt原子/分子的寡核苷酸加合物和具有两个Pt原子/分子的寡核苷酸加合物。仅含一个Pt（dien）2+的加合物的NMR谱表明，虽然存在两个鸟嘌呤碱，但仅形成一个加合物。这表明在分子中优选N7原子之一。
The products resulting from reaction of cis-Pt(NH3)2Cl2 with d(CpCpGpG), d(GpCpG), d(pCpGpCpG), d(pGpCpGpC) and d(CpGpCpG) and from reaction of [Pt(dien)Cl]Cl with d(CpCpGpG) and d(GpCpG) have been characterized with the aid of proton NMR spectroscopy, circular dichroic spectroscopy and Pt analysis. The binding sites of the Pt compounds were determined by pH-dependent NMR spectroscopy. Binding of the two Pt compounds invariably occurs at the guanine N7 atoms. In all compounds containing [cis-Pt(NH3)2]2+ chelates are formed by coordination of platinum to two guanines of the same oligonucleotide. The resulting intrastrand-cross-linked oligonucleotides contain either d(GpG) . cisPt units, or d(GpCpG) . cisPt units. In the latter case the middle cytosine is not coordinated to platinum. As a result the conformational changes originating from these two chelates are different from each other. In the case of [Pt(dien)Cl]Cl as a starting product, two types of oligonucleotide adducts are formed, i.e. those with one Pt atom/molecule and those with two Pt atoms/molecule. The NMR spectra of the adducts containing only one Pt(dien)2+ show that only one adduct is formed, although two guanine bases are present. This indicates a preference for one of the N7 atoms in the molecule.
使用核酸外切酶 III 确定 DNA 确定序列中稳定损伤的位点：环丁烷嘧啶二聚体以及顺式和反式二氯二氨铂 II 示例。
影响因子：
14.9
作者：
Royer-Pokora,B;Gordon,LK;Haseltine,WA
通讯作者：
Haseltine,WA