Sil overexpression in lung cancer characterizes tumors with increased mitotic activity
Sil overexpression in lung cancer characterizes tumors with increased mitotic activity
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DOI：
10.1038/sj.onc.1207685
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发表时间：
2004-07-08
期刊：
影响因子：
8
通讯作者：
Izraeli, S
中科院分区：
文献类型：
--
作者：
Erez, A;Perelman, M;Izraeli, S
SIL(SCL Interrupting Locus)基因是从T细胞急性淋巴细胞白血病最常见的染色体重排中克隆出来的。它是一种即刻早期基因，其表达与细胞增殖有关。SIL蛋白水平在细胞周期中受到严格调控，在有丝分裂时达到峰值，在过渡到G1时消失。最近的一项研究发现，sIL是在原发腺癌中过度表达预测转移扩散的17个基因之一。我们推测sIL可能在癌症的发生中起作用。为了解决这个问题，我们使用了几种方法。利用多肿瘤组织阵列，我们发现sIL蛋白的表达主要在肺癌中增加，但在其他肿瘤的亚群中也有较低水平的表达。微阵列基因表达分析和肺癌样本的免疫组织化学证实了这些观察结果。肺癌组织中sIL基因的表达与几个动粒检验点基因的表达及组织病理学有丝分裂指数相关。Se观察表明，sIL基因的过度表达是有丝分裂活性增强的肿瘤的特征。
Sil (SCL interrupting locus) was cloned from the most common chromosomal rearrangement in T-cell acute lymphoblastic leukemia. It is an immediate early gene whose expression is associated with cell proliferation. Sil protein levels are tightly regulated during the cell cycle, reaching peak levels in mitosis and disappearing on transition to G1. A recent study found Sil to be one of 17 genes whose overexpression in primary adenocarcinomas predicts metastatic spread. We hypothesized that Sil might have a role in carcinogenesis. To address this question, we utilized several approaches. Using a multi-tumor tissue array, we found that Sil protein expression was increased mostly in lung cancer, but also at lower levels, in a subset of other tumors. Microarray gene expression analysis and immunohistochemistry of lung cancer samples verified these observations. Sil gene expression in lung cancer correlated with the expression of several kinetochore check-point genes and with the histopathologic mitotic index. The se observations suggest that overexpression of the Sil gene characterizes tumors with increased mitotic activity.