Phenotypic analysis of mice deficient in the type 2 galanin receptor (GALR2)
Phenotypic analysis of mice deficient in the type 2 galanin receptor (GALR2)
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DOI：
10.1128/mcb.25.11.4804-4811.2005
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发表时间：
2005-06-01
影响因子：
5.3
通讯作者：
Steiner, RA
中科院分区：
文献类型：
--
作者：
Gottsch, ML;Zeng, HK;Steiner, RA
甘丙肽是一种神经肽，参与摄食、生殖、认知、伤害感受和癫痫易感性的调节。已知甘丙肽受体（GALR）有三种亚型（GALR1、GALR2和GALR3），它们以不同的亲和力与甘丙肽结合，并在脑和外周神经系统中具有各自独特的分布、信号传导机制和推定的功能。为了进一步了解GALR2可能的生理意义，我们创建了GALR2缺陷的突变小鼠，并将其表型与野生型（WT）同窝或年龄匹配的对照组进行了比较，包括基本运动和感觉功能，摄食行为，生殖，情绪，学习和记忆以及癫痫易感性。表型分析显示，携带GALR2缺失的动物在任何测量变量上与其WT对照没有显著差异。我们的结论是，要么GALR2在这些生理功能中不起作用，要么通过冗余或补偿，这些突变动物可以适应GALR2的先天性缺失。也可以想象，GALR2在其中一些功能中仅起微妙的作用，并且其损失的影响无法通过本文使用的分析方法检测到。
Galanin is a neuropeptide implicated in the regulation of feeding, reproduction, cognition, nociception, and seizure susceptibility. There are three known galanin receptor (GALR) subtypes (GALR1, GALR2, and GALR3), which bind to galanin with different affinities and have their own unique distributions, signaling mechanisms, and putative functions in the brain and peripheral nervous system. To gain further insight into the possible physiological significance of GALR2, we created mutant mice that were deficient in GALR2 and compared their phenotype to that of wild-type (WT) littermate or age-matched controls, with respect to basic motor and sensory function, feeding behavior, reproduction, mood, learning and memory, and seizure susceptibility. Phenotypic analysis revealed that animals bearing a deletion of GALR2 did not differ significantly from their WT controls in any of the measured variables. We conclude that either GALR2 plays no role in these physiological functions or through redundancy or compensation these mutant animals can adapt to the congenital absence of GALR2. It is also conceivable that GALR2 plays only a subtle role in some of these functions and that the impact of its loss could not be detected by the analytical procedures used here.