Intracellular substrates of brain-enriched receptor protein tyrosine phosphatase rho (RPTPρ/PTPRT)
Intracellular substrates of brain-enriched receptor protein tyrosine phosphatase rho (RPTPρ/PTPRT)
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DOI：
10.1016/j.brainres.2006.07.122
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发表时间：
2006-10-20
期刊：
影响因子：
2.9
通讯作者：
Rotter, Andrej
中科院分区：
文献类型：
--
作者：
Besco, Julie A.;van Huijsduijnen, Rob Hooft;Rotter, Andrej
受体蛋白酪氨酸磷酸酶 rho (RPTPp/PTPRT) 是一种跨膜蛋白，在发育中和成人中枢神经系统中高度表达。它是 RPTP R2B 亚家族的成员，该亚家族包括 PTP kappa、PTP mu 和 PCP-2。谷胱甘肽-S-转移酶 (GST) 下拉实验显示 RPTP rho 与大脑中的几种粘附连接蛋白相互作用，包括 E-钙粘蛋白、N-钙粘蛋白、VE-钙粘蛋白 (cadherin-5)、桥粒芯糖蛋白、α、β 和 γ 连环蛋白、p120(ctn) 和 α-肌动蛋白。除 E-钙粘蛋白和 α-辅肌动蛋白外，高钠浓度下的结合显着降低。此外，免疫沉淀磷酸酶测定表明，E-钙粘蛋白以及较小程度的 p120(ctn) 被重组 RPTP rho 胞内片段酪氨酸去磷酸化，因此可能是 RPTP rho 的主要底物。 RPTP rho 与粘附连接成分的相互作用表明，这种磷酸酶可能将细胞外信号转导至肌动蛋白细胞骨架，从而在调节中枢神经系统中钙粘蛋白介导的细胞粘附中发挥作用。 (c) 2006 Elsevier B.V. 保留所有权利。
Receptor protein tyrosine phosphatase rho (RPTPp/PTPRT) is a transmembrane protein that is highly expressed in the developing and adult central nervous system. It is a member of the RPTP R2B subfamily, which includes PTP kappa, PTP mu and PCP-2. Glutathione-S-transferase (GST) pulldown assays were used to show that RPTP rho interacts with several adherens junctional proteins in brain, including E-cadherin, N-cadherin, VE-cadherin (cadherin-5), desmoglein, alpha, beta and gamma catenin, p120(ctn) and alpha-actinin. With the exception of E-cadherin and alpha-actinin, binding was considerably reduced at high sodium concentrations. Furthermore, immunoprecipitation phosphatase assays indicated that E-cadherin, and to a far lesser extent p120(ctn), were tyrosine dephosphorylated by a recombinant RPTP rho intracellular fragment, and thus, were likely to be primary substrates for RPTP rho. The interaction of RPTP rho with adherens junctional components suggests that this phosphatase may transduce extracellular signals to the actin cytoskeleton and thereby play a role in regulating cadherin-mediated cell adhesion in the central nervous system. (c) 2006 Elsevier B.V. All rights reserved.