A sensitive predictor for potential GPI lipid modification sites in fungal protein sequences and its application to genome-wide studies for Aspergillus nidulans, Candida albicans Neurospora crassa, Saccharomyces cerevisiae and Schizosaccharomyces pombe
A sensitive predictor for potential GPI lipid modification sites in fungal protein sequences and its application to genome-wide studies for Aspergillus nidulans, Candida albicans Neurospora crassa, Saccharomyces cerevisiae and Schizosaccharomyces pombe
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DOI：
10.1016/j.jmb.2004.01.025
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发表时间：
2004-03-19
影响因子：
5.6
通讯作者：
Eisenhaber, F
中科院分区：
文献类型：
--
作者：
Eisenhaber, B;Schneider, G;Eisenhaber, F
真菌转酰胺酶复合物执行糖基磷脂酰肌醇（GPI）脂质锚定的前体蛋白具有重叠，但不同的序列特异性相比，动物系统。因此，分类特异性预测工具的识别真菌序列中的C-末端信号是必要的。我们从文献和真菌蛋白质组中收集了一组真菌前体蛋白序列。虽然识别信号的一般四段方案也保持在真菌前体中，但在细节上存在分类群特异性。一个真菌大-II预测已开发用于评估查询序列一致性与真菌特异性识别信号的要求。该预测器的灵敏度接近90%。假阳性预测率在0.1%的范围内。真菌big-H工具成功预测了C. Nuoffer和同事，并认识到人PLAP C末端不是真菌转酰胺酶复合物的靶标。已经产生了五种真菌蛋白质的潜在GPI脂质锚定蛋白质的列表，并对命中物进行了功能分类。真菌大II预测WWW服务器以及前体列表可在http：//mendel上获得。imp.univie.ac.at/gpi（C）2004 Elsevier Ltd.保留所有权利。
The fungal transamidase complex that executes glycosylphosphatidylinositol (GPI) lipid anchoring of precursor proteins has overlapping but distinct sequence specificity compared with the animal system. Therefore, a taxon-specific prediction tool for the recognition of the C-terminal signal in fungal sequences is necessary. We have collected a learning set of fungal precursor protein sequences from the literature and fungal proteomes. Although the general four segment scheme of the recognition signal is maintained also in fungal precursors, there are taxon specificities in details. A fungal big-II predictor has been developed for the assessment of query sequence concordance with fungi-specific recognition signal requirements. The sensitivity of this predictor is close to 90'%. The rate of false positive prediction is in the range of 0.1%. The fungal big-H tool successfully predicts the Gas1 mutation series described by C. Nuoffer and co-workers, and recognizes that the human PLAP C terminus is not a target for the fungal transamidase complex. Lists of potentially GPI lipid anchored proteins for five fungal proteornes have been generated and the hits have been functionally classified. The fungal big-II prediction WWW server as well as precursor lists are available at http://mendel. imp.univie.ac.at/gpi /fungi/ gpi-fungi.html (C) 2004 Elsevier Ltd. All rights reserved.