Adsorption kinetics of high mobility group box 1 protein in a polyacrylonitrile hemofiltration membrane
Adsorption kinetics of high mobility group box 1 protein in a polyacrylonitrile hemofiltration membrane
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DOI：
10.1111/1744-9987.13489
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发表时间：
2020-04-21
影响因子：
1.9
通讯作者：
Nishida, Osamu
中科院分区：
文献类型：
--
作者：
Nakamura, Tomoyuki;Moriyama, Kazuhiro;Nishida, Osamu
高迁移率族蛋白B1（HMGB 1）被认为是脓毒症中典型的内源性危险细胞因子。我们以前报道，聚丙烯腈（AN 69 ST）膜快速吸附HMGB 1。在此，设计了体外血液滤过系统，以评估AN 69 ST膜的HMGB 1吸附能力、吸附位点和吸附机制。在血液滤过过程中重复加入HMGB 1 7次。每次添加后，观察到循环HMGB 1快速下降，无饱和迹象。通过免疫电子显微镜，使用高浓度的HMGB 1，在两个膜表面和主体层内观察到HMGB 1在滤膜上的存在。我们假设，肝素添加到膜表面或过滤速率将有助于吸附机制。我们无法测量肝素和过滤的影响。尽管膜太大而不能在μ g/mL HMGB 1条件下饱和，但我们的结果表明AN 69 ST膜具有可用于治疗脓毒症的稳健吸收能力。
The high mobility group box 1 protein (HMGB1) is recognized as a prototypical endogenous danger cytokine in sepsis. We previously reported that a polyacrylonitrile (AN69ST) membrane rapidly adsorbed HMGB1. Herein, an in vitro hemofiltration system was designed to assess the HMGB1 adsorption capacity, adsorption sites, and adsorption mechanism of the AN69ST membrane. HMGB1 was repeatedly added seven times during hemofiltration. A rapid decrease in circulating HMGB1 was observed after every addition with no sign of saturation. Presence of HMGB1 on the filter membrane was observed on both membrane surfaces and within the bulk layer using a high concentration of HMGB1 by immunoelectron microscopy. We hypothesized that the addition of heparin to the membrane surface or filtration rate would contribute to the adsorption mechanism. We could not measure the influence of heparin and filtration. Although the membrane was too large to saturate under the mu g/mL HMGB1 conditions, our results show that the AN69ST membrane has a robust absorption capacity that could be used to treat sepsis.