On the use of fast blue, fluoro-gold and diamidino yellow for retrograde tracing after peripheral nerve injury:: uptake, fading, dye interactions, and toxicity
On the use of fast blue, fluoro-gold and diamidino yellow for retrograde tracing after peripheral nerve injury:: uptake, fading, dye interactions, and toxicity
复制标题
DOI：
10.1016/s0165-0270(01)00532-5
复制
发表时间：
2002-04-15
影响因子：
3
通讯作者：
Molander, C
中科院分区：
文献类型：
--
作者：
Puigdellívol-Sánchez, A;Valero-Cabré, A;Molander, C
研究了三种逆行荧光染料用于追踪受损外周轴突的有用性。大鼠坐骨神经被双侧横切，近端分别短暂暴露于右侧的固蓝（FB）、氟金（FG）或二脒黄（DY），以及左侧的盐水中。然后重新缝合神经并使其再生。 3个月后的电生理测试显示示踪剂组之间诱发肌肉和神经动作电位的潜伏期和幅度相似。然后在原始损伤的远端切断神经并暴露于第二种（不同的）染料。五天后，对背根神经节 (DRG) 和脊髓腹角中逆行标记的神经元进行计数。对于 DRG 和腹角中的所有三种染料，第一个示踪剂标记的神经元数量相似，但 FG 除外，FG 标记的运动神经元较少。当用作第二示踪剂时，在某些实验情况下，DY 标记的神经元比 FG 和 FB 少。与对照相比，由第一和/或第二示踪剂标记的神经症患者总数减少了约30%。讨论了细胞死亡的贡献以及神经再生研究中不同可选示踪剂组合的贡献。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The usefulness of three retrograde fluorescent dyes for tracing injured peripheral axons was investigated. The rat sciatic was transected bilaterally and the proximal end briefly exposed to either Fast Blue (FB), Fluoro-Gold (FG) or to Diamidino Yellow (DY) on the right side, and to saline on the left side, respectively. The nerves were then resutured and allowed to regenerate. Electrophysiological tests 3 months later showed similar latencies and amplitudes of evoked muscle and nerve action potentials between tracer groups. The nerves were then cut distal to the original injury and exposed to a second (different) dye. Five days later, retrogradely labelled neurones were counted in the dorsal root ganglia (DRGs) and spinal cord ventral horn, The number of neurones labelled by the first tracer was similar for all three dyes in the DRG and ventral horn except for FG, which labelled fewer motoneurones. When used as second tracer, DY labelled fewer neurones than FG and FB in some experimental situations. The total number of neurotics labelled by the first and/or second tracer was reduced by about 30% compared with controls. The contributions of cell death as well as different optional tracer combinations for studies of nerve regeneration are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.