Fabrication and characterization of microwell array chemical sensors
Fabrication and characterization of microwell array chemical sensors
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DOI：
10.1021/ac001069s
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发表时间：
2001-06-01
影响因子：
7.4
通讯作者：
Pantano, P
中科院分区：
文献类型：
--
作者：
Bernhard, DD;Mall, S;Pantano, P
微孔阵列已在相干光纤束的远端面上制造。典型的微孔阵列包含类似于 3000 个经过化学蚀刻的单独光纤，单个微孔的深度约为 1 至 14 微米，宽度约为 22 微米，并部分填充化学传感（聚合物 + 染料）层，以产生微孔阵列传感器 (MMAS)。 MWAS 是使用技术上方便的光引发聚合反应制造的，其中每个微孔内固定有大约 2 至 10 微米厚的 pH 敏感或 O-2 敏感传感层。 pH传感层包含固定在可光聚合聚(乙烯醇)膜中的异硫氰酸荧光素-葡聚糖缀合物。 O-2-传感层包含包埋在透气的光聚合硅氧烷膜中的钌金属络合物。通过使用落射荧光显微镜/电荷耦合装置成像系统获取发光图像来定量pH和PO2。pH敏感的MWAS显示出类似于6.4的pK(a)和类似于2.5秒的响应时间。 O-2 敏感 MWAS 的行为符合非线性 Stern-Volmer 模型，最大 I-0/I 约为 4，响应时间约为 2.5 s，MWAS 的优势在于，适当大小的样品（例如单个生物细胞）可以与单个微孔中的传感基质共定位。
Microwell arrays have been fabricated on the distal face of coherent fiber-optic bundles. A typical microwell array comprises similar to 3000 individual optical fibers that were etched chemically, Individual microwells were similar to1 to 14-mum deep with similar to 22-mum widths and were filled partially with a chemical sensing (polymer + dye) layer to produce a microwell array sensor (MMAS). MWASs were fabricated using a technically expedient, photoinitiated polymerization reaction whereby a similar to2 to 10-mum thick pH-sensitive or O-2-sensitive sensing layer was immobilized inside each microwell. The pH-sensing layer comprised fluorescein isothiocyanate-dextran conjugate immobilized in a photopolymerizable poly(vinyl alcohol) membrane. The O-2-sensing layer comprised a ruthenium metal complex entrapped in a gas-permeable photopolymerizable siloxane membrane. pH and PO2 were quantitated by acquiring luminescence images using an epifluorescence microscope/charge-coupled device imaging system, The pH-sensitive MWAS displayed a pK(a) of similar to6.4 and a response time of similar to2.5 s. The O-2-sensitive MWAS behaved according to a nonlinear Stern-Volmer model with a maximum I-0/I of similar to4 and a response time of similar to2.5 s, MWASs are advantageous in that suitably sized samples such as single biological cells can be co-localized with the sensing matrix in individual microwells.