Resistance gene analogs are conserved and clustered in soybean
Resistance gene analogs are conserved and clustered in soybean
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DOI：
10.1073/pnas.93.21.11746
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发表时间：
1996-10-15
影响因子：
11.1
通讯作者：
Shoemaker, RC
中科院分区：
文献类型：
--
作者：
Kanazin, V;Marek, LF;Shoemaker, RC
从广泛的植物类群中克隆的抗性基因序列揭示了序列同源性和结构基序的显着相似性。这在赋予对病毒、细菌和真菌病原体的抗性的基因中观察到。本研究利用抗病基因N（烟草）、RPS 2（拟南芥）和L 6（亚麻）编码区保守序列设计的寡核苷酸引物，从大豆[Glycine max（L.）Merr.]。扩增产物的测序表明，至少有9类抗性基因类似物（RGA）被检测到。这些类的成员的遗传图谱将它们定位到八个不同的连锁群。几个RGA位点定位在已知抗性基因附近。利用8种RGA的特异性引物和探针筛选了大豆DNA的细菌人工染色体文库，并鉴定了含有7种RGA特有序列的克隆。单个细菌人工染色体含有2-10个单一RGA类的成员。RGA类成员的聚类和序列相似性表明了它们进化的共同过程。我们的数据表明，它可能是可能的，使用克隆的抗性基因的保守基序的序列同源性，以确定候选的抗性位点，从广泛不同的植物类群。
Sequences of cloned resistance genes from a wide range of plant taxa reveal significant similarities in sequence homology and structural motifs. This is observed among genes conferring resistance to viral, bacterial, and fungal pathogens. In this study, oligonucleotide primers designed for conserved sequences from coding regions of disease resistance genes N (tobacco), RPS2 (Arabidopsis) and L6 (flax) were used to amplify related sequences from soybean [Glycine max (L.) Merr.]. Sequencing of amplification products indicated that at least nine classes of resistance gene analogs (RGAs) were detected. Genetic mapping of members of these classes located them to eight different linkage groups. Several RGA loci mapped near known resistance genes. A bacterial artificial chromosome library of soybean DNA was screened using primers and probes specific for eight RGA classes and clones were identified containing sequences unique to seven classes. Individual bacterial artificial chromosomes contained 2-10 members of single RGA classes. Clustering and sequence similarity of members of RGA classes suggests a common process in their evolution. Our data indicate that it may be possible to use sequence homologies from conserved motifs of cloned resistance genes to identify candidate resistance loci from widely diverse plant taxa.