DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression
DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression
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DNA介导的多重耐药性转移和质膜糖蛋白表达
DOI：
10.1128/mcb.2.8.881-889.1982
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发表时间：
1982
影响因子：
5.3
通讯作者：
V. Ling
中科院分区：
文献类型：
--
作者：
Paul G. Debenham;N. Kartner;Louis Siminovitch;R. John;Riordan;V. Ling
秋水仙碱耐药的中国仓鼠卵巢(CHO)细胞突变体，其耐药性是由于药物通透性降低而导致的，我们的实验室以前已经分离到这种突变株。这种渗透性的降低影响了广泛的无关药物，导致突变株表现出多重耐药表型。已鉴定出一个170,000道尔顿的细胞表面糖蛋白(P-糖蛋白)，其表达似乎与耐药程度有关。在本研究中，我们通过DNA介导的基因转移将秋水仙素耐药突变株的DNA转移到敏感的小鼠L细胞上，使其具有多重耐药表型。用抗突变型CHO细胞膜的抗血清染色，可在这些DNA转化子的质膜上检测到P-糖蛋白。这些结果与P-糖蛋白表达与多重耐药表型之间的因果关系是一致的。
Colchicine-resistant Chinese hamster ovary (CHO) cell mutants whose resistance results from reduced drug permeability have been isolated previously in our laboratories. This reduced permeability affects a wide range of unrelated drugs, resulting in the mutants displaying a multiple drug resistance phenotype. A 170,000-dalton cell surface glycoprotein (P-glycoprotein) was identified, and its expression appears to correlate with the degree of resistance. In this study we were able to confer the multiple drug resistance phenotype on sensitive mouse L cells by DNA-mediated gene transfer of DNA obtained from the colchicine-resistant mutants. P-glycoprotein was detected in plasma membranes of these DNA transformants by staining with an antiserum raised against membranes of mutant CHO cells. These results are consistent with a causal relationship between P-glycoprotein expression and the multiple drug resistance phenotype.