Integrated biomarker responses in zebrafish exposed to sulfonamides
Integrated biomarker responses in zebrafish exposed to sulfonamides
复制标题
暴露于磺胺类药物的斑马鱼的综合生物标志物反应
DOI：
10.1016/j.etap.2014.07.020
复制
发表时间：
2014-09-01
影响因子：
4.3
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Lin, Tao;Yu, Shilin;Chen, Wei
磺胺类药物等分散的药物对水生生态系统构成威胁。我们使用扩展的斑马鱼（Danio rerio）毒性试验评估了磺胺暴露的潜在生物标志物。磺胺类药物对斑马鱼的自发游泳活动和心率有明显的影响。研究了斑马鱼暴露于磺胺类药物（磺胺甲恶唑、磺胺嘧啶和磺胺二甲嘧啶）后谷胱甘肽S-转移酶（GST）和丙二醛（MDA）的变化。GST和MDA均表现出对磺胺暴露的时间依赖性反应。磺胺类药物染毒3 d后，GST活性显著升高，MDA含量在染毒第1 d达到最大值，随后下降。这些结果表明，MDA可能是一个更敏感的生物标志物磺胺毒性比GST。这些研究表明，SDZ是一种典型的代谢酶诱导剂，表明它对水生生态系统构成潜在的生态毒理学风险。(C)2014爱思唯尔有限公司版权所有。
Dispersed pharmaceuticals such as sulfonamides pose a threat to aquatic ecosystems. We evaluated potential biomarkers of sulfonamide exposure using an extended zebrafish (Danio rerio) toxicity test. The tested sulfonamides induced obvious effects on spontaneous swimming activity and heartbeat rate in zebrafish. Glutathione S-transferase (GST) and malondialdehyde (MDA) were examined to reflect the biomarker response of zebrafish exposed to three sulfonamides (sulfamethoxazole, sulfadiazine (SDZ) and sulfadimidine). Both GST and MDA showed time-dependent responses to sulfonamide exposure. GST activity was significantly increased after exposure to sulfonamides for 3 days, while MDA concentration reached a maximum during the first day and then declined. These results suggest that MDA may be a more sensitive biomarker of sulfonamide toxicity than GST. These investigations demonstrated that SDZ was a typical inducer of metabolic enzymes, suggesting that it poses a potential ecotoxicological risk to aquatic ecosystems. (C) 2014 Elsevier B.V. All rights reserved.