Exhaled ethane -: An in vivo biomarker of lipid peroxidation in interstitial lung diseases
Exhaled ethane -: An in vivo biomarker of lipid peroxidation in interstitial lung diseases
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DOI：
10.1378/chest.128.4.2387
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发表时间：
2005-10-01
期刊：
影响因子：
9.6
通讯作者：
Motoyoshi, K
中科院分区：
文献类型：
--
作者：
Kanoh, S;Kobayashi, H;Motoyoshi, K
背景：氧化应激在间质性肺疾病（ILD）的发病机制和进展中发挥作用。呼出的乙烷是脂质过氧化的产物，已被提议作为体内氧化应激的生物标志物。目的：确定ILD患者呼出的乙烷水平是否升高，并将其与其他临床参数进行比较。从34例ILD患者中采集呼吸样本，包括13例特发性肺纤维化（IPF）患者，9例隐源性机化性肺炎患者，胶原血管病相关性间质性肺炎6例，肺结节病6例。在入院时和3周后采集气体样本。结果：ILD患者呼出的乙烷水平（n = 34，平均值± SD，8.5 ± 8.0 pmol/dL）高于健康志愿者（n = 16，2.9 ± 1.0 pmol/dL; p < 0.001）。系列测量显示，乙烷水平的增加和减少在很大程度上与临床病程一致。4例具有持续高乙烷水平的IPF患者死亡或恶化，而乙烷水平< 5.0 pmol/dL的患者保持稳定或改善。呼出乙烷浓度与乳酸脱氢酶（斯皮尔曼等级相关系数[rs]，0.28，p = 0.026）和C反应蛋白（rs，0.38，p = 0.025）水平呈正相关，与Pao（2）呈负相关（rs，- 0.40，p = 0.0026）。Ga-67放射性造影显示摄取增加的患者表现出更高的乙烷水平（n = 19，7.5 +/- 5.7 pmol/dL），与造影未显示摄取增加的患者相比（n = 10，3.0 +/- 2.4 pmol/dL; p < 0.01）。呼出乙烷在ILD患者中升高，并与临床结局相关，表明其提供了有关持续氧化应激的有用信息，从而提供了ILD中疾病活动和严重程度的信息。
Background: Oxidative stress plays a role in the pathogenesis and progression of interstitial lung disease (ILD). Exhaled ethane is a product of lipid peroxidation that has been proposed as a biomarker of oxidative stress in vivo.Objectives: To determine whether the exhaled ethane level is elevated in patients with ILD and to compare it with other clinical parameters.Methods: Breath samples were collected from 34 patients with ILD, including 13 with idiopathic pulmonary fibrosis (IPF), 9 patients with cryptogenic organizing pneumonia, 6 patients with collagen vascular disease-associated interstitial pneumonia, and 6 patients with pulmonary sarcoidosis. Gas samples were obtained at hospital admission and after 3 weeks. After each expired sample was concentrated using a trap-and-purge procedure, the ethane level was analyzed by gas chromatography.Results: Exhaled ethane levels were elevated in ILD patients (n = 34, mean +/- SD, 8.5 +/- 8.0 pmol/dL) compared with healthy volunteers (n = 16, 2.9 +/- 1.0 pmol/dL; p < 0.001). Serial measurements revealed that increase and decrease of ethane levels were largely consistent with the clinical course. Four patients with IPF who had persistently high ethan e levels died or deteriorated, whereas those with ethane levels < 5.0 pmol/dL remained stable or improved. Exhaled ethane concentrations were positively correlated with levels of lactate dehydrogenase (Spearman rank correlation coefficient [rs], 0.28, p = 0.026) and C-reactive protein (rs, 0.38, p = 0.025) and were inversely correlated with Pao(2) (rs, - 0.40, p = 0.0026). Patients showing increased uptake on Ga-67 scintigraphy demonstrated higher ethane levels (n = 19, 7.5 +/- 5.7 pmol/dL) compared with those who did not show increased uptake on scintigraphy (n = 10, 3.0 +/- 2.4 pmol/dL; p < 0.01).Conclusions: Exhaled ethane is elevated in patients with ILD and is correlated with the clinical outcome, suggesting that it provides useful information about ongoing oxidative stress, and thereby disease activity and severity in ILD.