Large-scale synthesis of ultrathin Au-Pt nanowires assembled on thionine/graphene with high conductivity and sensitivity for electrochemical immunosensor
Large-scale synthesis of ultrathin Au-Pt nanowires assembled on thionine/graphene with high conductivity and sensitivity for electrochemical immunosensor
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大规模合成组装在硫堇/石墨烯上的超薄金铂纳米线，具有高电导率和高灵敏度，用于电化学免疫传感器
DOI：
10.1016/j.electacta.2014.03.065
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发表时间：
2014-06-01
影响因子：
6.6
通讯作者：
Qian, Weiping
中科院分区：
文献类型：
--
作者：
Lu, Wenbo;Ge, Juan;Qian, Weiping
本文首次研制了一种基于超薄Au-铂纳米线修饰的硫氨酸/氧化石墨烯(AuPtNWs/THI/rGO)的新型无标记固有电活性氧化还原生物传感器，用于检测癌胚抗原(CEA)。超薄的AuPtNW是通过一锅法合成的，不使用任何稳定剂或模板。以表面官能化的THI/rGO为载体，配位负载超薄的AuPtNWs，得到了AuPtNWs/THI/rGO复合材料。AuPtNWs/THI/rGO复合材料不仅有利于抗体的固定化，而且有利于电子传递。结果表明，所制备的AuPtNWs/THI/rGO复合材料可以作为一种灵敏的无标记电化学免疫传感器用于CEA的测定。在优化的条件下，该免疫传感器的线性范围为50fg.mL(-1)~100ng.mL.(-1)(R=0.998)，在信噪比为3时，检测下限为6fg.mL.制备的CEA免疫传感器具有较高的灵敏度、重复性和稳定性。我们的研究表明，所提出的免疫传感器也成功地用于测定稀释的血液样本中的CEA。(C)2014爱思唯尔有限公司。保留所有权利。
In this article, for the first time, a novel, label-free and inherent electroactive redox biosensor based on ultrathin Au-Pt nanowire-decorated thionine/reduced graphene oxide (AuPtNWs/THI/rGO) is developed for carcinoembryonic antigen (CEA) detection. Ultrathin AuPtNWs are prepared by a one-pot synthesis method without the use of any stabilizer or template. The AuPtNWs/THI/rGO composites are obtained by the THI/rGO composites surface functionalized with -NH2 group employed as a support for loading ultrathin AuPtNWs by coordination. The AuPtNWs/THI/rGO composites not only favor the immobilization of antibody but also facilitate the electron transfer. It is found that the resultant AuPtNWs/THI/rGO composites can be designed to act as a sensitive label-free electrochemical immunosensor for CEA determination. Under the optimized conditions, the linear range of the proposed immunosensor is estimated to be from 50 fg.mL(-1) to 100 ng.mL(-1) (R= 0.998) and the detection limit is estimated to be 6 fg center dot mL(-1) at a signal-to-noise ratio of 3, respectively. The prepared immunosensor for detection of CEA shows high sensitivity, reproducibility and stability. Our study demonstrates that the proposed immunosensor has also been used to determine CEA successfully in diluted blood samples. (C) 2014 Elsevier Ltd. All rights reserved.