A voltage sensor-domain protein is a voltage-gated proton channel
A voltage sensor-domain protein is a voltage-gated proton channel
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DOI：
10.1126/science.1122352
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发表时间：
2006-04-28
期刊：
影响因子：
56.9
通讯作者：
Okamura, Y
中科院分区：
文献类型：
--
作者：
Sasaki, M;Takagi, M;Okamura, Y
电压门控质子通道已被广泛观察到，但尚未在分子水平上确定。在这里，我们报道了一个类似于电压门控离子通道的电压传感器域的四跨膜蛋白是电压门控质子通道。过表达该蛋白的细胞表现出去极化诱导的向外电流并伴有尾电流。在质子的平衡势处发生电流反转。电流表现出ph依赖性门控和锌离子敏感性，这是电压门控质子通道的两个特征。电压依赖性对序列变化的反应表明，小鼠电压传感器域蛋白本身是一个通道，而不是另一个通道蛋白的调节器。
Voltage-gated proton channels have been widely observed but have not been identified at a molecular level. Here we report that a four-transmembrane protein similar to the voltage-sensor domain of voltage-gated ion channels is a voltage-gated proton channel. Cells overexpressing this protein showed depolarization-induced outward currents accompanied by tail currents. Current reversal occured at equilibrium potentials for protons. The currents exhibited pH-dependent gating and zinc ion sensitivity, two features which are characteristic of voltage-gated proton channels. Responses of voltage dependence to sequence changes suggest that mouse voltage-sensor domain-only protein is itself a channel, rather than a regulator of another channel protein.