Mechanical strain increases type I collagen expression in pulmonary fibroblasts in vitro.
Mechanical strain increases type I collagen expression in pulmonary fibroblasts in vitro.
复制标题
机械应变增加体外肺成纤维细胞中 I 型胶原蛋白的表达。
DOI：
10.1152/jappl.2000.88.1.203
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Breen,EC
中科院分区：
文献类型：
--
作者：
Breen,EC
组织重塑是对肺内机械张力的适应性反应。然而，肺成纤维细胞在这一反应中的作用还没有得到很好的表征。本研究探讨细胞外基质对成纤维细胞机械应变反应的影响。将细胞培养在涂有纤维连接蛋白、层粘连蛋白或弹性蛋白的柔性底面上，并暴露于应变中。在这些条件下，成纤维细胞垂直于力矢量对齐。这种刺激导致在层粘连蛋白或弹性蛋白上培养的细胞中α-1(I)前胶原基因的表达增加，但不能增加纤维连接蛋白。α-1(I)前胶原m RNA在暴露后6 h开始升高，72 h时达到对照水平，[~(3 H)]-Pro掺入新合成的前胶原反应了m RNA水平的变化。在层粘连蛋白或弹性蛋白上培养的成纤维细胞分别比未经应变的细胞多掺入190%和114%的[~3H]Pro进入前胶原。在纤维连接蛋白上培养的成纤维细胞中，没有发现差异。这些结果表明，成纤维细胞在体外对机械应变有反应，这种反应是通过细胞-细胞外基质相互作用来传递的。
Tissue remodeling is an adaptive response to mechanical tension in the lung. However, the role of pulmonary fibroblasts in this response has not been well characterized. This study investigates the influence of extracellular matrix on the response of fibroblasts to mechanical strain. Cells were cultured on flexible-bottom surfaces coated with fibronectin, laminin, or elastin and exposed to strain. Under these conditions, fibroblasts align perpendicular to the force vector. This stimulus results in an increase in α1(I) procollagen mRNA in cells cultured on laminin or elastin but not fibronectin. Increased α1(I) procollagen mRNA was detected 6 h after exposure to strain and reached control levels by 72 h. [3H]proline incorporation into newly synthesized procollagen reflects changes in mRNA levels. Strained fibroblasts cultured on laminin or elastin incorporated 190 and 114%, respectively, more [3H]proline into procollagen than did unstrained cells. No difference was detected in strained fibroblasts cultured on fibronectin. These results suggest that fibroblasts respond to mechanical strain in vitro,and this response is signaled by cell-extracellular matrix interactions.