Carbon nanotube-coating accelerated cell adhesion and proliferation on poly (L-lactide)
Carbon nanotube-coating accelerated cell adhesion and proliferation on poly (L-lactide)
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DOI：
10.1016/j.apsusc.2012.01.012
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发表时间：
2012-12
影响因子：
6.7
通讯作者：
E. Hirata;T. Akasaka;M. Uo;H. Takita;F. Watari;A. Yokoyama
中科院分区：
文献类型：
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作者：
E. Hirata;T. Akasaka;M. Uo;H. Takita;F. Watari;A. Yokoyama
将多壁碳纳米管(MWCNTs)包覆在聚乳酸(PLLA)表面，以改善其表面性质。此外，还对其表面特性和细胞培养性能进行了检测。整个PLLA表面均匀地覆盖着多壁碳纳米管，保持了独特的管状结构。MWCNT包覆的PLLA比未包覆的PLLA具有更高的润湿性。人成骨肉瘤细胞株(Saos2)在CNT包被的PLLA上附着良好，而未包被的PLLA在种植后2小时有少量细胞附着。在第1天和第3天，未包被的PLLA上的细胞数量仍然少于MWCNT包被的PLLA。此外，在第1天和第3天，附着在MWCNT包被的PLLA上的细胞的DNA含量显著高于未包被的PLLA(p<；P<0.05)。以DNA含量归一化的ALP活性在两种支架之间没有显著差异(p>；=0.1)。因此，在PLLA表面包覆MWCNT改善了表面的润湿性和细胞的早期附着。
The surface of a polylactic acid (PLLA) was coated multiwalled carbon nanotubes (MWCNTs) in order to improve the surface properties. In addition, its surface characteristics and cell culturing properties were examined. Whole surface of PLLA was homogeneously covered by MWCNTs maintained a unique tubular structure. MWCNT-coated PLLA showed remarkable higher wettability than uncoated PLLA. Human osteosarcoma cell line (Saos2) adhered well on the CNT-coated PLLA whereas there are few cells attached on the uncoated PLLA at 2 h after seeding. The number of the cells on uncoated PLLA was still smaller than on the MWCNT-coated PLLA at 1 and 3 days. Moreover, The DNA content in the cells attached to the MWCNT-coated PLLA was significantly higher than that on the uncoated PLLA (p< 0.05) at 1 and 3 days. There was no significant difference between the scaffolds for ALP activity normalized by DNA content at both term (p> 0.1). Therefore MWCNT-coating on PLLA improved the surface wettability and initial cell attachment at early stage.