Analysis of changes in acute-phase plasma proteins in an acute inflammatory response and in rheumatoid arthritis using two-dimensional gel electrophoresis
Analysis of changes in acute-phase plasma proteins in an acute inflammatory response and in rheumatoid arthritis using two-dimensional gel electrophoresis
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DOI：
10.1002/elps.1150190234
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发表时间：
1998-02-01
期刊：
影响因子：
2.9
通讯作者：
Anderson, NL
中科院分区：
文献类型：
--
作者：
Doherty, NS;Littman, BH;Anderson, NL
用二维凝胶分析检测了正常人、类风湿性关节炎(RA)患者与正常人、类风湿性关节炎(RA)患者和服用替尼达普(120 Mg)和吡罗昔康(20 Mg)治疗的RA患者急性炎症反应(肠外伤寒疫苗接种)前后19种血浆蛋白水平的差异。在RA患者中，血清淀粉样蛋白A(SAA)、结合珠蛋白α(2)、结合珠蛋白α(1)、结合珠蛋白α(2)、结合珠蛋白β(1)、结合珠蛋白α(2)、结合珠蛋白β、α(1)-抗凝乳蛋白酶和C3激活物水平升高，而载脂蛋白A-I、载脂蛋白A-IV、转甲状腺蛋白、GC-球蛋白、α(2)-HS糖蛋白、α(2)-巨球蛋白和(1)-B糖蛋白水平降低。与吡罗昔康相比，替尼达普降低了RA患者的α(1)-抗蛋白酶和SAA水平，而升高了转甲素、GC-球蛋白、α(2)-HS-糖蛋白和α(2)-巨球蛋白的水平。病人。C反应蛋白(CRP)不能在2-D凝胶上定量，但当用速率散射比浊法测量时，与吡罗昔康相比，替尼达普治疗后水平降低。对于接种伤寒疫苗的急性炎症反应，急性期蛋白反应的一般模式类似于慢性炎症条件下的RA。替尼达普对RA患者急性期蛋白阳性和阴性的影响与吡罗昔康明显不同。
Two-dimensional (2-D) gel analysis was used to examine differences in the levels of 19 plasma proteins: before and after an acute inflammatory reaction (parenteral typhoid vaccination) in normal subjects, between rheumatoid arthritis (RA) patients and normals and in RA patients treated with tenidap (120 mg) and piroxicam (20 mg). Typhoid vaccination increased levels of SAA, haptoglobin alpha(1), haptoglobin alpha(2), haptoglobin beta and alpha(1)-anti-chymotrypsin but decreased transthyretin and apolipoprotein E. In RA patients, serum amyloid A (SAA), haptoglobin alpha(2), haptoglobin beta, alpha(1)-antichymotrypsin and C3 proactivator levels were elevated while apolipoprotein A-I, apolipoprotein A-IV, transthyretin, Gc-globulin, alpha(2)-HS glycoprotein, alpha(2)-macroglobulin and alpha(1)-B glycoprotein levels were decreased, compared to normals. Compared to piroxicam, tenidap lowered levels of alpha(1)-antiprotease and SAA but raised the levels of transthyretin, Gc-globulin, alpha(2)-HS-glycoprotein and alpha(2)-macroglobulin in RA. patients. C-reactive protein (CRP) could not be quantified on 2-D gels but, when measured by rate nephelometry, levels were reduced after treatment with tenidap compared to piroxicam. The general pattern of the acute phase protein response to an acute inflammatory response to typhoid vaccination is similar to that in the chronic inflammatory condition, RA. The impact of tenidap on both positive and negative acute-phase proteins in RA patients could clearly be distinguished from that of piroxicam.