EMBRYONIC EXPRESSION OF THE SINGLE TRIBOLIUM ENGRAILED HOMOLOG
EMBRYONIC EXPRESSION OF THE SINGLE TRIBOLIUM ENGRAILED HOMOLOG
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DOI：
10.1002/dvg.1020150103
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
DENELL, RE
中科院分区：
文献类型：
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作者：
BROWN, SJ;PATEL, NH;DENELL, RE
我们已经克隆并测序了果蝇enrailed基因的单一赤拟谷盗同源物。预测的蛋白质包含一个同源框和几个结构域之间的所有enrailed的基因迄今为止确定的保守。此外，它还包含了一些特定于果蝇和果蝇的感染同源物的特征，这表明这些特征很可能存在于全变态昆虫共同祖先的祖先基因中。我们使用交叉反应的单克隆抗体，4D 9，跟踪Engrailed蛋白在拟谷盗胚胎分割过程中的表达。与其他昆虫一样，Engrailed沿着每个节的后缘沿着积累在细胞核中。第一条雕纹出现在胚胎雏形凝聚时。然后，当原基伸长成胚带时，在每个片段形成的形态学证据之前，出现了由前到后的条纹。与果蝇（一种长胚昆虫）一样，拟谷盗（一种短胚昆虫）的engraled基因的表达是由几个同源的分节基因的表达决定的，这表明不同的发育类型具有相似的遗传调控机制。(C)1994 Wiley-Liss，Inc.
We have cloned and sequenced the single Tribolium homolog of the Drosophila engrailed gene. The predicted protein contains a homeobox and several domains conserved among all engrailed genes identified to date. In addition it contains several features specific to the invected homologs of Bombyx and Drosophila, indicating that these features most likely were present in the ancestral gene in the common ancestor of holometabolous insects. We used the cross-reacting monoclonal antibody, 4D9, to follow the expression of the Engrailed protein during segmentation in Tribolium embryos. As in other insects, Engrailed accumulates in the nuclei of cells along the posterior margin of each segment. The first Engrailed stripe appears as the embryonic rudiment condenses. Then as the rudiment elongates into a germ band, Engrailed stripes appear in an anterior to posterior progression, just prior to morphological evidence of the formation of each segment. As in Drosophila (a long germ insect), expression of engrailed in Tribolium (classified as a short germ insect) is preceeded by the expression of several homologous segmentation genes, sug gesting that similar genetic regulatory mechanisms are shared by diverse developmental types. (C) 1994 Wiley-Liss, Inc.